Increased phosphorylation of a specific protein in mitogen-stimulated lymphocytes

Alvin M. Malkinson; Tingchung Wang; John E. Foker

doi:10.1016/0014-4827(78)90387-7

Increased phosphorylation of a specific protein in mitogen-stimulated lymphocytes

Alvin M. Malkinson, Tingchung Wang, John E. Foker

Surgery

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The phosphorylation of proteins from murine splenic lymphocytes was studied. When the phosphorylation of proteins in extracts from Concanavalin A (ConA)-treated lymphocytes was compared with that of resting lymphocytes, there was only one detectable difference between them. A protein of 135 000 mol. wt was highly phosphorylatable in extracts from ConA-treated cells while phosphate incorporation into this protein was slight in extracts from untreated cells. This effect could be observed 12 h after ConA treatment and was maximal during S phase. This soluble protein was also phosphorylated in intact lymphocytes.

Original language	English (US)
Pages (from-to)	442-445
Number of pages	4
Journal	Experimental Cell Research
Volume	113
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(78)90387-7
State	Published - May 1978

Bibliographical note

Funding Information:
We thank Carolyn E. McSwigan and Cathy Marquardt for their excellent technicala ssistance.T his work was supportedb y a National Foundation-Basil O’Connor Grant, USPHS grants GM 22492a nd CA 16228a, nd a National Leukemia Association grant.

Publisher link

10.1016/0014-4827(78)90387-7

Find It

Find It at U of M Libraries

Cite this

@article{38eb8d83e6274d049fba0237ca2cbf1a,

title = "Increased phosphorylation of a specific protein in mitogen-stimulated lymphocytes",

abstract = "The phosphorylation of proteins from murine splenic lymphocytes was studied. When the phosphorylation of proteins in extracts from Concanavalin A (ConA)-treated lymphocytes was compared with that of resting lymphocytes, there was only one detectable difference between them. A protein of 135 000 mol. wt was highly phosphorylatable in extracts from ConA-treated cells while phosphate incorporation into this protein was slight in extracts from untreated cells. This effect could be observed 12 h after ConA treatment and was maximal during S phase. This soluble protein was also phosphorylated in intact lymphocytes.",

author = "Malkinson, {Alvin M.} and Tingchung Wang and Foker, {John E.}",

note = "Funding Information: We thank Carolyn E. McSwigan and Cathy Marquardt for their excellent technicala ssistance.T his work was supportedb y a National Foundation-Basil O{\textquoteright}Connor Grant, USPHS grants GM 22492a nd CA 16228a, nd a National Leukemia Association grant.",

year = "1978",

month = may,

doi = "10.1016/0014-4827(78)90387-7",

language = "English (US)",

volume = "113",

pages = "442--445",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Increased phosphorylation of a specific protein in mitogen-stimulated lymphocytes

AU - Malkinson, Alvin M.

AU - Wang, Tingchung

AU - Foker, John E.

N1 - Funding Information: We thank Carolyn E. McSwigan and Cathy Marquardt for their excellent technicala ssistance.T his work was supportedb y a National Foundation-Basil O’Connor Grant, USPHS grants GM 22492a nd CA 16228a, nd a National Leukemia Association grant.

PY - 1978/5

Y1 - 1978/5

N2 - The phosphorylation of proteins from murine splenic lymphocytes was studied. When the phosphorylation of proteins in extracts from Concanavalin A (ConA)-treated lymphocytes was compared with that of resting lymphocytes, there was only one detectable difference between them. A protein of 135 000 mol. wt was highly phosphorylatable in extracts from ConA-treated cells while phosphate incorporation into this protein was slight in extracts from untreated cells. This effect could be observed 12 h after ConA treatment and was maximal during S phase. This soluble protein was also phosphorylated in intact lymphocytes.

AB - The phosphorylation of proteins from murine splenic lymphocytes was studied. When the phosphorylation of proteins in extracts from Concanavalin A (ConA)-treated lymphocytes was compared with that of resting lymphocytes, there was only one detectable difference between them. A protein of 135 000 mol. wt was highly phosphorylatable in extracts from ConA-treated cells while phosphate incorporation into this protein was slight in extracts from untreated cells. This effect could be observed 12 h after ConA treatment and was maximal during S phase. This soluble protein was also phosphorylated in intact lymphocytes.

UR - http://www.scopus.com/inward/record.url?scp=0018140934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018140934&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(78)90387-7

DO - 10.1016/0014-4827(78)90387-7

M3 - Article

C2 - 299654

AN - SCOPUS:0018140934

SN - 0014-4827

VL - 113

SP - 442

EP - 445

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Increased phosphorylation of a specific protein in mitogen-stimulated lymphocytes

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this