Abstract
The phosphorylation of proteins from murine splenic lymphocytes was studied. When the phosphorylation of proteins in extracts from Concanavalin A (ConA)-treated lymphocytes was compared with that of resting lymphocytes, there was only one detectable difference between them. A protein of 135 000 mol. wt was highly phosphorylatable in extracts from ConA-treated cells while phosphate incorporation into this protein was slight in extracts from untreated cells. This effect could be observed 12 h after ConA treatment and was maximal during S phase. This soluble protein was also phosphorylated in intact lymphocytes.
Original language | English (US) |
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Pages (from-to) | 442-445 |
Number of pages | 4 |
Journal | Experimental Cell Research |
Volume | 113 |
Issue number | 2 |
DOIs | |
State | Published - May 1978 |
Bibliographical note
Funding Information:We thank Carolyn E. McSwigan and Cathy Marquardt for their excellent technicala ssistance.T his work was supportedb y a National Foundation-Basil O’Connor Grant, USPHS grants GM 22492a nd CA 16228a, nd a National Leukemia Association grant.