Acutely, nerve growth factor (NGF) exerts profound effects on nociceptive transmission and produces pain and hyperalgesia. In the present study, we sought to determine the tissue levels and role of NGF after a plantar incision. A substantial increase in NGF protein expression occurred in skin 4-h, 1-day and 2-days and 5-days after incision comparing contralateral uninjured skin. Plantar incision did not change NGF levels in the tibial nerve and L4-L6 dorsal root ganglia. The therapeutic effect of a monoclonal antibody against endogenous NGF was evaluated by intraperitoneal administration of a single preoperative dose of anti-NGF. Of three different doses of anti-NGF used, the highest dose 2.8 mg/kg anti-NGF attenuated or almost abolished guarding pain scores at 4-h, 1-day (>80% decrease) and 2-days after incision. This effect is dose dependent in that lower doses (1, 0.1 mg/kg) were effective only at 1-day after incision. Anti-NGF also attenuated heat hyperalgesia at 1-day and 2-days after incision when the highest dose was used. However, treatment by anti-NGF did not affect C-fibers sensitized 1-day after incision in a glabrous skin-tibial nerve in vitro preparation. In conclusion, increased NGF was present in skin after plantar incision. NGF contributes to some incision-induced pain behaviors, guarding and heat hyperalgesia. Anti-NGF did not affect the extent of sensitization of C-fibers observed in vitro.
|Original language||English (US)|
|Number of pages||9|
|State||Published - Sep 2005|
Bibliographical noteFunding Information:
The authors indebted to Dr David Shelton (Rinat Neurosciences, Palo Alto, CA) for generous gift of anti-NGF antibody. We thank Kenneth Lamb for technical assistance and Philippe Richebe for critical reading of our manuscript. This work was supported by the Department of Anesthesia at the University of Iowa and by National Institutes of Health, Bethesda, Maryland grants GM 55831 and GM 067762 to T.J.B. T.J.B. is a consultant for Rinat Neurosciences.