Increased monokines in cytomegalovirus infected myelomonocytic cell cultures

Lloyd W. Turtinen, Aris Assimacopoulos, Ashley T. Haase

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

In an investigation of the role of monokines in CMV associated immunosuppression we document modulation of both TNF and IL-1β in CMV infected THP-1 cells. CMV infected cultures released almost two-fold more IL-1β protein and contained significantly higher IL-1β mRNA levels than uninfected cultures for 72-96 h after induction. In both CMV infected and uninfected cultures, significant amounts of IL-1β protein were not detected until 24 h post induction, while maximum amounts of TNF were detected in culture supernatants by 3 h post induction, suggesting that TNF may play a role in IL-1β induction. TNF levels subsequently declined but in infected cultures remained over 2.5-fold higher than controls through 96 h. The CMV alteration in the kinetics and extent of IL-1β release must be indirectly mediated by CMV since only 1% of THP-1 cells were infected. Most infected cells expressed CMV immediate early proteins but did not overexpress IL-1. We speculate that CMV infected cells release excess TNF or other stimulatory factors which increase IL-1β synthesis. Since IL-1β is increased, the decreased IL-1 'activity' described by others as an explanation in part for the immunosuppressive effects of infection may actually reflect alterations of IL-1 inhibitor levels during CMV infection.

Original languageEnglish (US)
Pages (from-to)135-145
Number of pages11
JournalMicrobial Pathogenesis
Volume7
Issue number2
DOIs
StatePublished - Aug 1989

Keywords

  • THP-1 cells
  • human cytomegalovirus
  • interleukin 1β
  • monocytes
  • tumor necrosis factor

Fingerprint Dive into the research topics of 'Increased monokines in cytomegalovirus infected myelomonocytic cell cultures'. Together they form a unique fingerprint.

Cite this