Increased expression of vascular endothelial growth factor in neo-vascularized canine brain tissue

Chi Ho Yu, Ji Young Yhee, Jong Hyuk Kim, KeumSoon Im, Na Hyun Kim, So Young Kwon, Tai Young Hur, Jung Hyang Sur

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Abstract

This retrospective study was done to characterize the levels of vascular endothelial growth factor (VEGF) and hypoxia inducible factor 1 (HIF-1a) in dog brains with neo-vascularization in the cerebral cortex of frontal, temporal, and parietal lobe by using immunohistochemistry (IHC) and Western blot. In neo-vascularized (NV) brains, we analyzed the number and area of blood vessels and the expression of VEGF and HIF-1a. The IHC results showed that the number and area of blood vessels, as assessed by immunolabeling for von Willebrand factor, was higher in the NV brain than in the control brain. The Western blot results showed that the level of VEGF was increased, predominantly in NV brain of the cerebral cortex relative to the clinically normal cerebral cortex, whereas the expression of HIF-1a in NV brains was not different from the control brains. Our study showed that dilatation of vessels and development of new vessels in the cerebral cortex were observed in cases of canine CNS disease and found increased expression of VEGF in canine brains with neo-vascularization.

Original languageEnglish (US)
Pages (from-to)62-68
Number of pages7
JournalCanadian Journal of Veterinary Research
Volume76
Issue number1
StatePublished - Jan 13 2012

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Yu, C. H., Yhee, J. Y., Kim, J. H., Im, K., Kim, N. H., Kwon, S. Y., Hur, T. Y., & Sur, J. H. (2012). Increased expression of vascular endothelial growth factor in neo-vascularized canine brain tissue. Canadian Journal of Veterinary Research, 76(1), 62-68.