Abstract
The purpose of this study was to determine the sphingolipid (SL) profile in cells defective in autophagy protein ATG-7 and overall cell death after photodynamic therapy (PDT) with the photosensitizer Pc 4. MCF-7 human breast cancer cells with downregulated ATG-7 and their scrambled controls (Scr) were used. Exposure of ATG-7 knockdown cells to PDT led to increased cell killing. PDT evoked an early (2 h) greater global increase in ceramides in ATG-7 defective cells compared to Scr cells. The total increases in dihydroceramide (DHceramide) were significant at 2 and 24 h in both cell types post-PDT. The levels of sphingosine-1-phosphate (S1P) and sphingosine were decreased below resting levels at both time points irrespective of the cell type. The data imply that ceramide might be a marker of ATG-7 deficiency in cells sensitized to PDT.
Original language | English (US) |
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Pages (from-to) | 101-105 |
Number of pages | 5 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 494 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1 2010 |
Keywords
- Autophagy
- Ceramide
- Dihydroceramide
- Photodynamic therapy