Increased arterial stiffness and extracellular matrix reorganization in intrauterine growth-restricted fetal sheep

Reuben Blair Dodson, Paul J. Rozance, Bradley S. Fleenor, Carson C. Petrash, Lauren G. Shoemaker, Kendall S. Hunter, Virginia L. Ferguson

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Background:Fetal intrauterine growth restriction (IUGR) results in increased placental resistance to blood flow, fetal hypertension, and increased pulsatility stresses shown to lead to vascular remodeling. We tested our hypothesis that IUGR causes decreased compliance in the carotid and umbilical arteries due to altered extracellular matrix (ECM) composition and structure.Methods:A sheep model of placental insufficiency-induced IUGR (PI-IUGR) was created by exposure of the pregnant ewe to elevated ambient temperatures. Umbilical and carotid arteries from near-term fetuses were tested with pressure-diameter measurements to compare passive compliance in control and PI-IUGR tissues. ECM composition was measured via biochemical assay, and the organization was determined by using histology and second-harmonic generation imaging.Results:We found that PI-IUGR increased arterial stiffness with increased collagen engagement, or transition stretch. PI-IUGR carotid arteries exhibited increased collagen and elastin quantity, and PI-IUGR umbilical arteries exhibited increased sulfated glycosaminoglycans. Histomorphology showed altered collagen-to-elastin ratios with altered cellular proliferation. Increased stiffness indicates altered collagen-to-elastin ratios with less elastin contribution leading to increased collagen engagement.Conclusion:Because vessel stiffness is a significant predictor in the development of hypertension, disrupted ECM deposition in IUGR provides a potential link between IUGR and adult hypertension.

Original languageEnglish (US)
Pages (from-to)147-154
Number of pages8
JournalPediatric Research
Issue number2
StatePublished - Feb 2013


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