Inclusion of antibodies to cell culture media preserves the integrity of genes encoding rl13 and the pentameric complex components during fibroblast passage of human cyto7megalovirus

Amine Ourahmane, Xiaohong Cui, Li He, Meaghan Catron, Dirk P. Dittmer, Ahmed Al Qaffasaa, Mark R. Schleiss, Laura Hertel, Michael A. McVoy

Research output: Contribution to journalArticle

Abstract

Propagation of human cytomegalovirus (CMV) in cultured cells results in genetic adaptations that confer improved growth in vitro and significant attenuation in vivo. Mutations in RL13 arise quickly, while mutations in the UL128-131A locus emerge later during fibroblast passage and disrupt formation of a glycoprotein complex that is important for entry into epithelial and endothelial cells. As CMV replicates in the context of host antibodies in vivo, we reasoned that antibodies might mitigate the accumulation of adaptive mutations during cell culture passage. To test this, CMV in infant urine was used to infect replicate fibroblast cultures. One lineage was passaged in the absence of CMV-hyperimmuneglobulin (HIG) while the other was passaged with HIG in the culture medium. The former lost epithelial tropism and acquired mutations disrupting RL13 and UL131A expression, whereas the latter retained epithelial tropism and both gene loci remained intact after 22 passages. Additional mutations resulting in single amino acid changes also occurred in UL100 encoding glycoprotein M, UL102 encoding a subunit of the helicase/primase complex, and UL122 encoding the Immediate Early 2 protein. An epitheliotropic RL13+/UL131A+ virus was isolated by limiting dilution in the presence of HIG and expanded to produce a working stock sufficient to conduct cell tropism experiments. Thus, production of virus stocks by culture in the presence of antibodies may facilitate in vitro experiments using viruses that are genetically more authentic than previously available.

Original languageEnglish (US)
Article number221
JournalViruses
Volume11
Issue number3
DOIs
StatePublished - Mar 2019
Externally publishedYes

Fingerprint

Cytomegalovirus
Tropism
Culture Media
Cell Culture Techniques
Fibroblasts
Mutation
Antibodies
Viruses
Genes
Glycoproteins
Immediate-Early Proteins
DNA Primase
Cultured Cells
Endothelial Cells
Epithelial Cells
Urine
Amino Acids
Growth
In Vitro Techniques

Keywords

  • Adaptation
  • Antibodies
  • Cell culture
  • Cytomegalovirus

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

Cite this

Inclusion of antibodies to cell culture media preserves the integrity of genes encoding rl13 and the pentameric complex components during fibroblast passage of human cyto7megalovirus. / Ourahmane, Amine; Cui, Xiaohong; He, Li; Catron, Meaghan; Dittmer, Dirk P.; Qaffasaa, Ahmed Al; Schleiss, Mark R.; Hertel, Laura; McVoy, Michael A.

In: Viruses, Vol. 11, No. 3, 221, 03.2019.

Research output: Contribution to journalArticle

Ourahmane, Amine ; Cui, Xiaohong ; He, Li ; Catron, Meaghan ; Dittmer, Dirk P. ; Qaffasaa, Ahmed Al ; Schleiss, Mark R. ; Hertel, Laura ; McVoy, Michael A. / Inclusion of antibodies to cell culture media preserves the integrity of genes encoding rl13 and the pentameric complex components during fibroblast passage of human cyto7megalovirus. In: Viruses. 2019 ; Vol. 11, No. 3.
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