INCENP and Aurora B Promote Meiotic Sister Chromatid Cohesion through Localization of the Shugoshin MEI-S332 in Drosophila

Tamar D. Resnick, David L. Satinover, Fiona MacIsaac, P. Todd Stukenberg, William C. Earnshaw, Terry L. Orr-Weaver, Mar Carmena

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The chromosomal passenger complex protein INCENP is required in mitosis for chromosome condensation, spindle attachment and function, and cytokinesis. Here, we show that INCENP has an essential function in the specialized behavior of centromeres in meiosis. Mutations affecting Drosophila incenp profoundly affect chromosome segregation in both meiosis I and II, due, at least in part, to premature sister chromatid separation in meiosis I. INCENP binds to the cohesion protector protein MEI-S332, which is also an excellent in vitro substrate for Aurora B kinase. A MEI-S332 mutant that is only poorly phosphorylated by Aurora B is defective in localization to centromeres. These results implicate the chromosomal passenger complex in directly regulating MEI-S332 localization and, therefore, the control of sister chromatid cohesion in meiosis.

Original languageEnglish (US)
Pages (from-to)57-68
Number of pages12
JournalDevelopmental Cell
Volume11
Issue number1
DOIs
StatePublished - Jul 1 2006

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Chromatids
Meiosis
Chromosomes
Drosophila
Aurora Kinase B
Centromere
Condensation
Proteins
Chromosome Segregation
Cytokinesis
Substrates
Mitosis
Mutation

Keywords

  • CELLCYCLE
  • DNA

Cite this

INCENP and Aurora B Promote Meiotic Sister Chromatid Cohesion through Localization of the Shugoshin MEI-S332 in Drosophila. / Resnick, Tamar D.; Satinover, David L.; MacIsaac, Fiona; Stukenberg, P. Todd; Earnshaw, William C.; Orr-Weaver, Terry L.; Carmena, Mar.

In: Developmental Cell, Vol. 11, No. 1, 01.07.2006, p. 57-68.

Research output: Contribution to journalArticle

Resnick, Tamar D. ; Satinover, David L. ; MacIsaac, Fiona ; Stukenberg, P. Todd ; Earnshaw, William C. ; Orr-Weaver, Terry L. ; Carmena, Mar. / INCENP and Aurora B Promote Meiotic Sister Chromatid Cohesion through Localization of the Shugoshin MEI-S332 in Drosophila. In: Developmental Cell. 2006 ; Vol. 11, No. 1. pp. 57-68.
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