The chromosomal passenger complex protein INCENP is required in mitosis for chromosome condensation, spindle attachment and function, and cytokinesis. Here, we show that INCENP has an essential function in the specialized behavior of centromeres in meiosis. Mutations affecting Drosophila incenp profoundly affect chromosome segregation in both meiosis I and II, due, at least in part, to premature sister chromatid separation in meiosis I. INCENP binds to the cohesion protector protein MEI-S332, which is also an excellent in vitro substrate for Aurora B kinase. A MEI-S332 mutant that is only poorly phosphorylated by Aurora B is defective in localization to centromeres. These results implicate the chromosomal passenger complex in directly regulating MEI-S332 localization and, therefore, the control of sister chromatid cohesion in meiosis.
Bibliographical noteFunding Information:
We thank Richard R. Adams and David M. Glover for gifts of antisera and Daniel Roth for technical assistance to M.C. Laura Lee carried out the initial mapping studies on QA26. Some fly strains were obtained from the Bloomington Stock Center. T.D.R. and T.L.O.-W. were supported by National Science Foundation grant MCB0132237 and National Institutes of Health (NIH) grant GM39341. T.D.R. was supported by an Anna Fuller graduate fellowship. D.L.S. was supported by training grant HD07528 for Developmental Biology at the University of Virginia. P.T.S. was supported by a grant from the NIH, GM63045, and by a grant from the Pew Charitable Trust. M.C. and W.C.E. were supported by the Wellcome Trust, of which W.C.E. is a Principal Research Fellow.