Inability of HL-60 cells induced by phorbol myristate acetate to produce macrophage-associated glycosaminoglycans

S. D. Luikart

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Glycosaminoglycans (GAGs) have been implicated in many functions of human blood cells. Normal human monocytes grown on plastic culture dishes for five days display macrophage-like morphology and functional characteristics concurrent with the appearance of a 4,6-disulfated chondroitin. This process has been regarded as in vitro differentiation. HL-60 human promyelocytic leukemia cells produce chondroitin 4-sulfate, which is the major GAG constitutent of normal granuloyctes and monocytes. These cells can be induced by phorbol myristate acetate (PMA) to develop many macrophage-like characteristics. This study investigates GAG production by HLA-60 cells after prolonged exposure to PMA. Disulfated chondroitin molecules were not detected. This biochemical abnormality may be an important factor in the functional deficiencies of these induced cells.

Original languageEnglish (US)
Pages (from-to)672-675
Number of pages4
JournalExperimental Hematology
Volume14
Issue number7
StatePublished - 1986

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