In vivo transfection of hepatitis C virus complementary DNA into rodent liver by asialoglycoprotein receptor mediated gene delivery

Masato Yamamoto, Norio Hayashi, Yasuhide Miyamoto, Tetsuo Takehara, Eiji Mita, Makoto Seki, Hideyuki Fusamoto, Takenobu Kamada

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


An in vivo model of hepatitis C virus (HCV) infection is needed to enable investigation of the mechanism of the liver injury that it causes. In this study, we used asialoglycoprotein receptor mediated gene delivery to obtain expression of the complementary DNA (cDNA) coding the core and part of the envelope 1 protein of HCV because selective delivery to the hepatocytes has been reported to be attained with this method. The optimum carrier-DNA ratio was examined using in vitro transfection and found to be important for the efficiency of this method. In transfection in vivo, microautoradiographical examination showed that the transfected plasmids were delivered selectively to the liver parenchymal cells. To obtain an immunohistochemically detectable level of protein expression in rodent liver, some modifications for increasing the in vivo transfection efficiency were performed; a lysosomal enzyme inhibitor, chloroquine, was used and the administration route of the carrier-DNA complex was changed from the tail vein to the portal vein. On the bases of these results, in vivo transfection with expression vector of HCV core/E1 region was performed. In rat liver transfected by intraportal injection with chloroquine, the transcript RNA and the core protein were detected. These results indicated that the HCV core/E1 expression vector was not merely delivered but also successfully expressed in the liver using asialoglycoprotein receptor mediated gene delivery. The number of the HCV core expressing cells in the transfected liver was similar to that in patients with hepatitis C. These in vivo transfected animals should be useful for investigating the role of this region in the liver injury caused by HCV.

Original languageEnglish (US)
Pages (from-to)847-855
Number of pages9
Issue number3
StatePublished - Sep 1995

Bibliographical note

Funding Information:
Abbreviations: HCV, hepatitis C virus; cDNA, complementary DNA; CTL, cytotoxic T lymphocyte; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; AsOR, asialo-orosomucoid; PBS, phosphate-buffered saline; dCTP, deoxycytidine triphosphate; PCR, polymerase chain reaction; Ig, immunoglobulin; RT, reverse transcription. From the 1First Department of Medicine, Osaka University School of Medicine, Osaka; and 2Biesciences Laboratory, Yokohama Research Center, Mitsu-bishi Chemical Corporation, Yokohama, Japan. Received August 15, 1994; accepted April 6, 1995. Supported by a grant-in-aid from the Ministry of Education, Science and Culture of Japan, Tokyo, Japan. Address reprint requests to: Norio Hayashi, MD, First Department of Medicine, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565, Japan. Copyright © 1995 by the American Association for the Study of Liver Diseases. 0270-9139/95/2203-002353.00/0


Dive into the research topics of 'In vivo transfection of hepatitis C virus complementary DNA into rodent liver by asialoglycoprotein receptor mediated gene delivery'. Together they form a unique fingerprint.

Cite this