We investigated whether adenovirus or adeno-associated virus vectors can transduce cerebellar Purkinje cells (PCs) in vivo. M ice were injected in the deep cerebellar nuclei (DCN) with lacZ-transducing adenovirus (Ad.RSV-βgal) or a recombinant AAV serotype 2 (rAAV2) vector (vTR-CM Vβ) mixed with wild-type adenovirus type 5 (Ad5). One week later, Ad.RSV-βgal transduced cells were found throughout the cerebellar white matter in a dose-dependent manner, but few transduced PCs were evident. In contrast, vTR-CM Vβ with Ad5 transduced several hundred PCs throughout the injected hemisphere. Using an rAAV2 vector transducing a CM V-regulated green fluorescent protein gene, we again found PC transduction, but only with Ad5 coinjection. To assess the effect of injection site and to determine whether the apparent requirement for Ad5 coinfection is observed with other promoters, a β-actin-regulated vector was injected with or without Ad5 to DCN or cerebellar cortical sites. Thousands of transduced PCs were observed under each condition. Cortical injection yielded greater numbers of transduced cells. Injection of rAAV2 without AdS led to greater specificity for PC transduction. We conclude that injection of rAAV2 vectors into the cerebellum is an effective means for transferring genes into substantial numbers of Purkinje cells in vivo.
Bibliographical noteFunding Information:
The authors thank Sergei Zolotukhin and Nicholas Muzyczka of the Gene Therapy Center, Department of Molecular Genetics and Microbiology, University of Florida (Gainesville, FL) for graciously providing the vTR-UF5 and vTR-UF11 used. This investigation was supported in part by National Research Service Award 5 F31 MH11640 from the National Institute of Mental Health to W.F.K., P01 HD32652, and the Lyle French Fund.
- Adeno-associated virus
- Deep cerebellar nuclei
- Gene transfer
- In vivo
- Purkinje cells