In vivo targeting of cell death using a synthetic fluorescent molecular probe

Bryan A. Smith; Shuzhang Xiao; William Wolter; James Wheeler; Mark A. Suckow; Bradley D. Smith

doi:10.1007/s10495-011-0601-5

In vivo targeting of cell death using a synthetic fluorescent molecular probe

Bryan A. Smith, Shuzhang Xiao, William Wolter, James Wheeler, Mark A. Suckow, Bradley D. Smith

Administration (AHS)

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

A synthetic, near-infrared, fluorescent probe, named PSS-794 was assessed for its ability to detect cell death in two animal models. The molecular probe contains a zinc(II)-dipicolylamine (Zn₂₊-DPA) affinity ligand that selectively targets exposed phosphatidylserine on the surface of dead and dying cells. The first animal model used rats that were treated with dexamethasone to induce thymic atrophy. Ex vivo fluorescence imaging and histological analysis of excised organs showed thymus uptake of PSS-794 was four times higher than a control fluorophore that lacked the Zn₂₊-DPA affinity ligand. In addition, the presence of PSS-794 produced a delayed and higher build up of dead and dying cells in the rat thymus. The second animal model employed focal beam radiation to induce cell death in tumor-bearing rats. Whole-body and ex vivo imaging showed that the amount of PSS-794 in a radiationtreated tumor was almost twice that in a non-treated tumor. The results indicate that PSS-794 may be useful for preclinical optical detection of tumor cell death due to therapy.

Original language	English (US)
Pages (from-to)	722-731
Number of pages	10
Journal	Apoptosis
Volume	16
Issue number	7
DOIs	https://doi.org/10.1007/s10495-011-0601-5
State	Published - Jul 2011

Bibliographical note

Funding Information:
Acknowledgments We thank Sarah Chapman for preparing the histology sections, and Chelsea Poschar and Samantha Korda for assistance in operating the accelerator. This work was supported by the Walther Cancer Foundation, the Notre Dame Integrated Imaging Facility, and NIH Grants R01GM059078 (B.D.S.), T32GM075762 (B.A.S.).

Keywords

Cell death
Molecular probe
Optical imaging
Tumor therapy
Zinc(II)-dipicolylamine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1007/s10495-011-0601-5

Find It

Find It at U of M Libraries

Cite this

@article{53bfc4fb6c524ba6a16e3ea58cf45c3f,

title = "In vivo targeting of cell death using a synthetic fluorescent molecular probe",

abstract = "A synthetic, near-infrared, fluorescent probe, named PSS-794 was assessed for its ability to detect cell death in two animal models. The molecular probe contains a zinc(II)-dipicolylamine (Zn2+-DPA) affinity ligand that selectively targets exposed phosphatidylserine on the surface of dead and dying cells. The first animal model used rats that were treated with dexamethasone to induce thymic atrophy. Ex vivo fluorescence imaging and histological analysis of excised organs showed thymus uptake of PSS-794 was four times higher than a control fluorophore that lacked the Zn2+-DPA affinity ligand. In addition, the presence of PSS-794 produced a delayed and higher build up of dead and dying cells in the rat thymus. The second animal model employed focal beam radiation to induce cell death in tumor-bearing rats. Whole-body and ex vivo imaging showed that the amount of PSS-794 in a radiationtreated tumor was almost twice that in a non-treated tumor. The results indicate that PSS-794 may be useful for preclinical optical detection of tumor cell death due to therapy.",

keywords = "Cell death, Molecular probe, Optical imaging, Tumor therapy, Zinc(II)-dipicolylamine",

author = "Smith, {Bryan A.} and Shuzhang Xiao and William Wolter and James Wheeler and Suckow, {Mark A.} and Smith, {Bradley D.}",

note = "Funding Information: Acknowledgments We thank Sarah Chapman for preparing the histology sections, and Chelsea Poschar and Samantha Korda for assistance in operating the accelerator. This work was supported by the Walther Cancer Foundation, the Notre Dame Integrated Imaging Facility, and NIH Grants R01GM059078 (B.D.S.), T32GM075762 (B.A.S.).",

year = "2011",

month = jul,

doi = "10.1007/s10495-011-0601-5",

language = "English (US)",

volume = "16",

pages = "722--731",

journal = "Apoptosis",

issn = "1360-8185",

publisher = "Springer Netherlands",

number = "7",

}

TY - JOUR

T1 - In vivo targeting of cell death using a synthetic fluorescent molecular probe

AU - Smith, Bryan A.

AU - Xiao, Shuzhang

AU - Wolter, William

AU - Wheeler, James

AU - Suckow, Mark A.

AU - Smith, Bradley D.

N1 - Funding Information: Acknowledgments We thank Sarah Chapman for preparing the histology sections, and Chelsea Poschar and Samantha Korda for assistance in operating the accelerator. This work was supported by the Walther Cancer Foundation, the Notre Dame Integrated Imaging Facility, and NIH Grants R01GM059078 (B.D.S.), T32GM075762 (B.A.S.).

PY - 2011/7

Y1 - 2011/7

N2 - A synthetic, near-infrared, fluorescent probe, named PSS-794 was assessed for its ability to detect cell death in two animal models. The molecular probe contains a zinc(II)-dipicolylamine (Zn2+-DPA) affinity ligand that selectively targets exposed phosphatidylserine on the surface of dead and dying cells. The first animal model used rats that were treated with dexamethasone to induce thymic atrophy. Ex vivo fluorescence imaging and histological analysis of excised organs showed thymus uptake of PSS-794 was four times higher than a control fluorophore that lacked the Zn2+-DPA affinity ligand. In addition, the presence of PSS-794 produced a delayed and higher build up of dead and dying cells in the rat thymus. The second animal model employed focal beam radiation to induce cell death in tumor-bearing rats. Whole-body and ex vivo imaging showed that the amount of PSS-794 in a radiationtreated tumor was almost twice that in a non-treated tumor. The results indicate that PSS-794 may be useful for preclinical optical detection of tumor cell death due to therapy.

AB - A synthetic, near-infrared, fluorescent probe, named PSS-794 was assessed for its ability to detect cell death in two animal models. The molecular probe contains a zinc(II)-dipicolylamine (Zn2+-DPA) affinity ligand that selectively targets exposed phosphatidylserine on the surface of dead and dying cells. The first animal model used rats that were treated with dexamethasone to induce thymic atrophy. Ex vivo fluorescence imaging and histological analysis of excised organs showed thymus uptake of PSS-794 was four times higher than a control fluorophore that lacked the Zn2+-DPA affinity ligand. In addition, the presence of PSS-794 produced a delayed and higher build up of dead and dying cells in the rat thymus. The second animal model employed focal beam radiation to induce cell death in tumor-bearing rats. Whole-body and ex vivo imaging showed that the amount of PSS-794 in a radiationtreated tumor was almost twice that in a non-treated tumor. The results indicate that PSS-794 may be useful for preclinical optical detection of tumor cell death due to therapy.

KW - Cell death

KW - Molecular probe

KW - Optical imaging

KW - Tumor therapy

KW - Zinc(II)-dipicolylamine

UR - http://www.scopus.com/inward/record.url?scp=80051546958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051546958&partnerID=8YFLogxK

U2 - 10.1007/s10495-011-0601-5

DO - 10.1007/s10495-011-0601-5

M3 - Article

C2 - 21499791

AN - SCOPUS:80051546958

SN - 1360-8185

VL - 16

SP - 722

EP - 731

JO - Apoptosis

JF - Apoptosis

IS - 7

ER -

In vivo targeting of cell death using a synthetic fluorescent molecular probe

Abstract

Bibliographical note

Keywords

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this