Reliable assessment of skeletal muscle strength is arguably the most important outcome measure in neuromuscular and musculoskeletal disease and injury studies, particularly when evaluating regenerative therapies' efficacy. Additionally, a critical aspect of translating many regenerative therapies is the demonstration of scalability and effectiveness in a large animal model. Various physiological preparations have been established to evaluate intrinsic muscle function properties in basic science studies, primarily in small animal models. The practices may be categorized as: in vitro (isolated fibers, fiber bundles, or whole muscle), in situ (muscle with intact vascularization and innervation but distal tendon attached to a force transducer), and in vivo (structures of the muscle or muscle unit remain intact). There are strengths and weaknesses to each of these preparations; however, a clear advantage of in vivo strength testing is the ability to perform repeated measurements in the same animal. Herein, the materials and methods to reliably assess isometric torque produced by the hindlimb dorsiflexor muscles in vivo in response to standard peroneal electrical stimulation in anesthetized pigs are presented.
|Original language||English (US)|
|Journal||Journal of Visualized Experiments|
|State||Published - 2021|
Bibliographical noteFunding Information:
Work and data presented were supported broadly by the US Army Medical Research and Material Command to BTC and SMG (#MR140099; #C_003_2015_USAISR; #C_001_2018_USAISR); and the Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development (I21 RX003188) to JAC and Dr. Luke Brewster. The authors gratefully acknowledge the USAISR Veterinary Service and Comparative Pathology Branches and UMN Advanced Preclinical Imaging Center for technical assistance in completing these studies.
The production of the video article and Open Access availability was sponsored by Aurora Scientific, Inc. Matthew Borkowski is employed by Aurora Scientific Inc. This company may potentially benefit from the research results.
© 2021 JoVE Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
PubMed: MeSH publication types
- Journal Article
- Research Support, U.S. Gov't, Non-P.H.S.
- Video-Audio Media