In vivo irreversible albumin-binding near-infrared dye conjugate as a naked-eye and fluorescence dual-mode imaging agent for lymph node tumor metastasis diagnosis

Wenjuan Zhang, Sichen Song, Hanxun Wang, Qiu Wang, Dan Li, Shunzhe Zheng, Zhuangyan Xu, Haotian Zhang, Jian Wang, Jin Sun

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Tumor metastases account for about 90% of cancer-related death, among which lymphatic metastases play a pivotal role. Therefore, high-efficiency sentinel lymph node (SLN) identification is significant for lymph node (LN) metastasis diagnosis in clinic. Herein, a novel in vivo covalent albumin-binding near-infrared (NIR) fluorescent IR820-maleimide conjugate (IR-Mal) is firstly designed as a SLN dual-mode imaging agent. The IR-Mal conjugate exhibits bright blue appearance and its large Stokes shift (over 100 nm) increases the fluorescent imaging resolution effectively. The fluorescence intensity of covalent albumin-binding IR-Mal (BSA-IR-Mal) complex is considerably stronger than that of IR-Mal. In vivo, IR-Mal could rapidly covalently bind the tissue interstitial albumin following subcutaneous administration and BSA-IR-Mal complexes could specifically accumulate on LN, and detect both normal and metastatic SLN through naked-eye and fluorescence imaging with high resolution. Moreover, the light stability and enhanced fluorescence intensity of BSA-IR-Mal complex facilitates its diagnosis accuracy. These findings suggest that such in vivo irreversible albumin-binding fluorescence conjugates could serve as a new agent for dual-mode imaging and have a great potential to be applied in the SLNs imaging and diagnosis.

Original languageEnglish (US)
Article number119279
JournalBiomaterials
Volume217
DOIs
StatePublished - Oct 2019

Bibliographical note

Funding Information:
This work was financially supported by the National Natural Science Foundation of China (No. 81573371 , U1608283 ), Key projects of Liaoning Province Department of Education (No. 2017LZD03 ), and Key projects of Technology bureau in Shenyang (No. 18400408 ).

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

  • Covalent albumin binding
  • IR820-maleimide conjugate
  • Lymph node metastasis diagnosis
  • Naked-eye and fluorescence dual-mode imaging

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