In vivo imaging of quantum dots encapsulated in phospholipid micelles

Benoit Dubertret, Paris Skourides, David J. Norris, Vincent Noireaux, Ali H. Brivanlou, Albert Libchaber

Research output: Contribution to journalArticlepeer-review

2710 Scopus citations

Abstract

Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this problem, we encapsulated individual nanocrystals in phospholipid block-copolymer micelles and demonstrated both in vitro and in vivo imaging. When conjugated to DNA, the nanocrystal-micelles acted as in vitro fluorescent probes to hybridize to specific complementary sequences. Moreover, when injected into Xenopus embryos, the nanocrystal-micelles were stable, nontoxic (<5 × 109 nanocrystals per cell), cell autonomous, and slow to photobleach. Nanocrystal fluorescence could be followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis.

Original languageEnglish (US)
Pages (from-to)1759-1762
Number of pages4
JournalScience
Volume298
Issue number5599
DOIs
StatePublished - Nov 29 2002

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