In vivo examination of membrane protein localization and degradation with green fluorescent protein

Randolph Y. Hampton, Ann Koning, Robin Wright, Jasper Rine

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.

Original languageEnglish (US)
Pages (from-to)828-833
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number2
DOIs
StatePublished - Jan 23 1996

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Green Fluorescent Proteins
Proteolysis
Hydroxymethylglutaryl CoA Reductases
Membrane Proteins
Proteins
Fungal Proteins
Membranes

Cite this

In vivo examination of membrane protein localization and degradation with green fluorescent protein. / Hampton, Randolph Y.; Koning, Ann; Wright, Robin; Rine, Jasper.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 2, 23.01.1996, p. 828-833.

Research output: Contribution to journalArticle

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