In vivo examination of membrane protein localization and degradation with green fluorescent protein

Randolph Y. Hampton, Ann Koning, Robin Wright, Jasper Rine

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.

Original languageEnglish (US)
Pages (from-to)828-833
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number2
DOIs
StatePublished - Jan 23 1996

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