In vitro T-cell generation from adult, embryonic, and induced pluripotent stem cells: Many roads to one destination

Michelle J. Smith, Beau R. Webber, Mahmood Mohtashami, Heather E. Stefanski, Juan Carlos Zúñiga-Pflücker, Bruce R. Blazar

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

T lymphocytes are critical mediators of the adaptive immune system and have the capacity to serve as therapeutic agents in the areas of transplant and cancer immunotherapy. While T cells can be isolated and expanded from patients, T cells derived in vitro from both hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (hPSCs) offer great potential advantages in generating a self-renewing source of T cells that can be readily genetically modified. T-cell differentiation in vivo is a complex process requiring tightly regulated signals; providing the correct signals in vitro to induce T-cell lineage commitment followed by their development into mature, functional, single positive T cells, is similarly complex. In this review, we discuss current methods for the in vitro derivation of T cells from murine and human HSPCs and hPSCs that use feeder-cell and feeder-cell-free systems. Furthermore, we explore their potential for adoption for use in T-cell-based therapies.

Original languageEnglish (US)
Pages (from-to)3174-3180
Number of pages7
JournalStem Cells
Volume33
Issue number11
DOIs
StatePublished - Nov 2015

Keywords

  • CD34-+-
  • Cell culture
  • Cord blood
  • Differentiation
  • Embryonic stem cells
  • Hematopoietic stem cells
  • Induced pluripotent stem cells
  • T cells

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Review

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