In vitro susceptibility of cytomegalovirus isolates from immunocompromised patients to acyclovir and ganciclovir

Nancy L. Cole, Henry H. Balfour

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Fifty-four isolates of cytomegalovirus (CMV) from 25 immunocompromised patients with CMV infections were examined in a plaque reduction assay to determine in vitro susceptibilities to both acyclovir and ganciclovir. Isolates were approximately 25-fold more sensitive to ganciclovir than to acyclovir. The mean ± SD ID50 for all isolates to acyclovir was 63.1 ± 30.2 μM, (median, 52.3 μM; range, 16.7-146.4 μM). The mean ID50 for all isolates to ganciclovir was 2.50 ± 1.27 μM, (median, 2.15 μM; range, 0.65-7.11 μM). Exposure to acyclovir or ganciclovir for periods of 2-5 wk did not alter the mean susceptibility of clinical isolates. However, one CMV strain isolated from a renal transplant patient after 14 days of acyclovir therapy displayed the lowest susceptibility of all strains tested (acyclovir 146.4 μM; ganciclovir 7.11 μM) and may represent selection of a resistant virus population.

Original languageEnglish (US)
Pages (from-to)255-261
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume6
Issue number3
DOIs
StatePublished - Mar 1987

Bibliographical note

Funding Information:
Supported by Grant AM 13083 from the NIH, and grants from the Minnesota Medical Foundation and Burroughs Wellcome Co.

Fingerprint Dive into the research topics of 'In vitro susceptibility of cytomegalovirus isolates from immunocompromised patients to acyclovir and ganciclovir'. Together they form a unique fingerprint.

Cite this