In vitro study of flavonoids, fatty acids, and steroids on proliferation of rat hepatic stellate cells

Farid A. Badria, Abdel Aziz A. Dawidar, Wael E. Houssen, Wayne T. Shier

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

There is a wealth of evidence that hepatic stellate cells (HSCs) orchestrate most of the important events in liver fibrogenesis. After liver injury, HSCs become activated to a profibrogenic myofibroblastic phenotype and can regulate net deposition of collagens and other matrix proteins in the liver. The proliferation of HSCs is mainly stimulated by the platelet-derived growth factor (PDGF). In this study, some compounds from natural resources have been tested for their activity to inhibit PDGF-driven proliferative activity of rat HSCs. Apigenin, quercetin, genistein, daidzin, and biochanin A exhibited > 75% inhibitory activity against HSC-T6. It was found that, γ-linolenic (γ-Ln), eicosapentanoic (EPA) and α- linolenic (α-Ln) acids showed a high inhibitory effect on proliferation of rat HSCs at 50 nmol/l. Cholest-4-ene-3,6-dione and stigmastone-4-en-3,6-dione are the most active steroids with inhibitory activities > 80% and this is most likely due to the presence of the 4-en-3,6-dione moiety in both compounds. These results revealed that the compounds which effectively blocked HSC proliferation may be beneficial in liver fibrosis. Structure-activity relationships (SAR) may provide a basis for rational structure modification.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume60
Issue number1-2
DOIs
StatePublished - 2005

Keywords

  • Hepatic Stellate Cells
  • Liver Fibrosis
  • Steroids

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