In vitro studies of corneal wound healing in dogs

Full thickness explants of dog cornea grown in organ culture demonstrate that the migration of epithelium and endothelium is mutually limited once contact between the two cell types has been established. When full thickness central corneal stab wounds have been made in vitro, the corneal epithelial cells will migrate into the wound and reach Descemet's membrane within 48 hr. There is no histological evidence of epithelial regression out of the anterior portion of the central stab wound or of stromal repair during the first 20 days in vitro under the conditions described.