In vitro inhibition of adhesion of enterotoxigenic Escherichia coli K88 to piglet intestinal mucus by egg-yolk antibodies

L. Z. Jin, Samuel K Baidoo, Ronald R. Marquardt, Andrew A. Frohlich

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

The objective of the study was to determine if the adhesion of E. coli K88 to piglet intestinal mucus could be inhibited in vitro by spray-dried egg-yolk anti-K88 antibodies. Binding of E. coli was monitored using a radioactive assay. Four 14±2-day-old healthy piglets were used for the preparation of mucus from the small intestine. Competition and displacement phenomena were investigated by incubating (a) egg-yolk antibodies and E. coli together prior to adding to the mucus and (b) E. coli and mucus, followed by egg-yolk antibodies. The results demonstrated that egg-yolk antibodies inhibited the adhesion of 3H-labeled local strain of hemolytic E. coli K88+ (E. coli K88+ MB) to piglet small intestinal mucus by 84.6-97.0% when the egg-yolk antibodies were diluted 10, 20, 40 or 100 times. The adhesion inhibiting effects of egg-yolk antibodies declined dramatically when the antibody dilution was more than 250-fold. A similar adhesion inhibiting effect was observed when egg-yolk antibodies were incubated with E. coli K88+ MB for 15, 30 and 60 min prior to the adhesion test. Egg-yolk antibodies when diluted 50- and 100-fold had a very strong inhibiting ability against E. coli K88+ MB at a concentration of 109 colony forming units (cfu) ml-1 (adhesion was <6%). However, dilution of 100 times for egg yolk antibodies was insufficient to inhibit the adhesion of E. coli K88+ MB to intestinal mucus when the concentration of E. coli K88+ MB was 1010 cfu ml-1. The displacement test indicated that there was no significant reduction in the adhesion of E. coli K88+ MB to the small intestinal mucus when egg-yolk antibodies were added after adhesion of the organism to the mucus. These studies demonstrate that anti-K88+ MB fimbriae antibodies from chicken egg-yolk when added to E. coli K88+ MB prevented their binding to receptors in the mucus isolated from the intestine of piglets. Copyright (C) 1998 Published by Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalFEMS Immunology and Medical Microbiology
Volume21
Issue number4
DOIs
StatePublished - Aug 1998

Keywords

  • Adhesion inhibition
  • E. coli K88
  • Egg-yolk antibody
  • Intestinal mucus receptor
  • Piglet

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