In vitro evolution of enzymes

Misha V. Golynskiy; John C. Haugner; Aleardo Morelli; Dana Morrone; Burckhard Seelig

doi:10.1007/978-1-62703-293-3_6

In vitro evolution of enzymes

Misha V. Golynskiy, John C. Haugner, Aleardo Morelli, Dana Morrone, Burckhard Seelig

Research output: Chapter in Book/Report/Conference proceeding › Chapter

13 Scopus citations

Abstract

In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.

Original language	English (US)
Title of host publication	Enzyme Engineering
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	73-92
Number of pages	20
ISBN (Print)	9781627032926
DOIs	https://doi.org/10.1007/978-1-62703-293-3_6
State	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	978
ISSN (Print)	1064-3745

Keywords

DNA display
Directed evolution
Enzyme
In vitro compartmentalization
In vitro selection
MRNA display
Ribosome display

Publisher link

10.1007/978-1-62703-293-3_6

Find It

Find It at U of M Libraries

Cite this

@inbook{1ac10eedd0e34a578d54f325689cb10b,

title = "In vitro evolution of enzymes",

abstract = "In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.",

keywords = "DNA display, Directed evolution, Enzyme, In vitro compartmentalization, In vitro selection, MRNA display, Ribosome display",

author = "Golynskiy, {Misha V.} and Haugner, {John C.} and Aleardo Morelli and Dana Morrone and Burckhard Seelig",

year = "2013",

doi = "10.1007/978-1-62703-293-3_6",

language = "English (US)",

isbn = "9781627032926",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "73--92",

booktitle = "Enzyme Engineering",

}

TY - CHAP

T1 - In vitro evolution of enzymes

AU - Golynskiy, Misha V.

AU - Haugner, John C.

AU - Morelli, Aleardo

AU - Morrone, Dana

AU - Seelig, Burckhard

PY - 2013

Y1 - 2013

N2 - In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.

AB - In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.

KW - DNA display

KW - Directed evolution

KW - Enzyme

KW - In vitro compartmentalization

KW - In vitro selection

KW - MRNA display

KW - Ribosome display

UR - http://www.scopus.com/inward/record.url?scp=84934437118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934437118&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-293-3_6

DO - 10.1007/978-1-62703-293-3_6

M3 - Chapter

C2 - 23423890

AN - SCOPUS:84934437118

SN - 9781627032926

T3 - Methods in Molecular Biology

SP - 73

EP - 92

BT - Enzyme Engineering

PB - Humana Press Inc.

ER -

In vitro evolution of enzymes

Abstract

Publication series

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this