In vitro evolution of enzymes

Misha V. Golynskiy, John C. Haugner, Aleardo Morelli, Dana Morrone, Burckhard Seelig

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.

Original languageEnglish (US)
Title of host publicationEnzyme Engineering
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages73-92
Number of pages20
ISBN (Print)9781627032926
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume978
ISSN (Print)1064-3745

Keywords

  • DNA display
  • Directed evolution
  • Enzyme
  • In vitro compartmentalization
  • In vitro selection
  • MRNA display
  • Ribosome display

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