In vitro efficacy of doxorubicin and etoposide against a feline injection site sarcoma cell line

Joseph Hill, Jessica Lawrence, Corey Saba, Michelle Turek, Brittany Feldhaeusser, Sheryl Coutermarsh-Ott, Jamie Barber, Jo Smith, Robert Gogal

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Feline injection site sarcoma (ISS) is a locally invasive tumor, in which surgical treatment is frequently combined with radiation or chemotherapy to improve tumor control. The focus of this study was to evaluate the cytotoxic effects of doxorubicin or etoposide on a feline injection site sarcoma cell line (JB) and to assess the impact of combining these drugs on cell death and cell cycle. Both single agent and combination drug administration increased cell death and significantly reduced the number of viable cells. Cells in G0/G1 were significantly reduced while the G2/M fraction was significantly increased following treatment. Collectively, combining doxorubicin and etoposide at the lower EC yielded comparable results to the EC50 of either drug alone in degree of cytotoxicity, level of apoptosis, and % of cells in G2/M. The results of this study indicate that doxorubicin and etoposide alone and in combination differentially alter ISS cell viability and cycle.

Original languageEnglish (US)
Pages (from-to)349-357
Number of pages9
JournalResearch in veterinary science
Volume97
Issue number2
DOIs
StatePublished - 2014

Bibliographical note

Funding Information:
Funding for this study was provided by the University of Georgia's Research Foundation .

Publisher Copyright:
© 2014 Elsevier Ltd.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • Apoptosis
  • Cell cycle
  • Doxorubicin
  • Etoposide
  • Injection site sarcoma

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