In vitro digestibility of individual amino acids in rumen-undegraded protein: The modified three-step procedure and the immobilized digestive enzyme assay

S. E. Boucher, S. Calsamiglia, C. M. Parsons, M. D. Stern, M. Ruiz Moreno, M. Vázquez-Añón, C. G. Schwab

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Three soybean meal, 3 SoyPlus (West Central Cooperative, Ralston, IA), 5 distillers dried grains with solubles, and 5 fish meal samples were used to evaluate the modified 3-step in vitro procedure (TSP) and the in vitro immobilized digestive enzyme assay (IDEA; Novus International Inc., St. Louis, MO) for estimating digestibility of AA in rumen-undegraded protein (RUP-AA). In a previous experiment, each sample was ruminally incubated in situ for 16 h, and in vivo digestibility of AA in the intact samples and in the rumen-undegraded residues (RUR) was obtained for all samples using the precision-fed cecectomized rooster assay. For the modified TSP, 5 g of RUR was weighed into polyester bags, which were then heat-sealed and placed into DaisyII incubator bottles. Samples were incubated in a pepsin/HCl solution followed by incubation in a pancreatin solution. After this incubation, residues remaining in the bags were analyzed for AA, and digestibility of RUP-AA was calculated based on disappearance from the bags. In vitro RUP-AA digestibility estimates obtained with this procedure were highly correlated to in vivo estimates. Corresponding intact feeds were also analyzed via the pepsin/pancreatin steps of the modified TSP. In vitro estimates of AA digestibility of the feeds were highly correlated to in vivo RUP-AA digestibility, which suggests that the feeds may not need to be ruminally incubated before determining RUP-AA digestibility in vitro. The RUR were also analyzed via the IDEA kits. The IDEA values of the RUR were good predictors of RUP-AA digestibility in soybean meal, SoyPlus, and distillers dried grains with solubles, but the IDEA values were not as good predictors of RUP-AA digestibility in fish meal. However, the IDEA values of intact feed samples were also determined and were highly correlated to in vivo RUP-AA digestibility for all feed types, suggesting that the IDEA value of intact feeds may be a better predictor of RUP-AA digestibility than the IDEA value of the RUR. In conclusion, the modified TSP and IDEA kits are good approaches for estimating RUP-AA digestibility in soybean meal products, distillers dried grains with solubles, and fish meal samples.

Original languageEnglish (US)
Pages (from-to)3939-3950
Number of pages12
JournalJournal of Dairy Science
Volume92
Issue number8
DOIs
StatePublished - Aug 2009

Bibliographical note

Funding Information:
The authors thank West Central (Ralston, IA), Adisseo (Alpharetta, GA), and Novus International Inc. (St. Louis, MO) for financial support of this project. Gratitude is also extended to Melissa Thrune (University of Minnesota, St. Paul) for help with the 3-step procedure, and Jennifer Wu (Novus International, St. Louis, MO) for help with the IDEA assays.

Keywords

  • Amino acid digestibility
  • Immobilized digestive enzyme assay
  • Modified three-step procedure

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