In vitro development of human Killer-Immunoglobulin Receptor-positive NK cells.

Frank Cichocki; Jeffrey S. Miller

doi:10.1007/978-1-60761-362-6_2

In vitro development of human Killer-Immunoglobulin Receptor-positive NK cells.

Frank Cichocki, Jeffrey S. Miller

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The in vitro culture system outlined in this chapter allows for the delineation of events that occur during the development of CD34(+) hematopoietic precursor cells into mature KIR(+) human NK cells. This system can also be utilized to study the effects of gene overexpression or knockdown on the process of NK cell differentiation through retroviral transduction and long-term culture. The necessary soluble factors and contact-dependent conditions for in vitro human NK cell development have been worked out in our laboratory over the past 16 years.

Original language	English (US)
Pages (from-to)	15-26
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	612
DOIs	https://doi.org/10.1007/978-1-60761-362-6_2
State	Published - 2010

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In vitro development of human Killer-Immunoglobulin Receptor-positive NK cells.

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