In vitro contractile studies within isolated tissue baths: Translational research from Visible Heart® Laboratories

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3 Scopus citations

Abstract

The isolated tissue bath research methodology was first developed in 1904. Since then, it has been recognized as an important tool in pharmacology and physiology research, including investigations into neuromuscular disorders. The tissue bath is still used routinely as the instrument for performing the “gold standard” test for clinical diagnosis of malignant hyperthermia susceptibility – the caffeine-halothane contracture test. Our research group has utilized this tool for several decades for a range of research studies, and we are currently one of four North American diagnostic centers for determining susceptibility for malignant hyperthermia. This review provides a brief summary of some of the historical uses of the tissue bath. Important experimental considerations for the operation of the tissue bath are further described. Finally, we discuss the different studies our group has performed using isolated tissue baths to highlight the broad potential applications.

Original languageEnglish (US)
Pages (from-to)584-597
Number of pages14
JournalExperimental Biology and Medicine
Volume247
Issue number7
DOIs
StatePublished - Apr 2022

Bibliographical note

Publisher Copyright:
© The Author(s) 2022.

Keywords

  • Organ bath
  • contracture testing
  • isolated tissue bath
  • malignant hyperthermia
  • muscle bath

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