In vitro circadian ANP secretion by gene transferring cells encapsulated in polycaprolactone tubes: Gene chronotherapy

Zhengrong Wang; Liguo Chen; Chaomin Wan; Yi Qu; Germaine G Cornelissen-Guillaume; F. Halberg

doi:10.1016/j.peptides.2004.05.014

In vitro circadian ANP secretion by gene transferring cells encapsulated in polycaprolactone tubes: Gene chronotherapy

Zhengrong Wang, Liguo Chen, Chaomin Wan, Yi Qu, Germaine G Cornelissen-Guillaume, F. Halberg

Physiology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A new insofar as chronobiologic therapeutic approach by atrial natriuretic peptide (ANP) for hypertension and/or congestive heart failure (CHF) is based on the release of ANP from ANP cDNA transfected Chinese Hamster Ovary (CHO) cells encapsulated in polycaprolactone (PCL) tubes. ANP secretion was maintained for at least 6 months. The encapsulated cells remained viable during culturing. Control cells without transferred ANP cDNA were negative. ANP secretion is circadian periodic, peaking around 04:18, shifted to around 07:56 by melatonin treatment. The encapsulation technique, based on principles of chronotherapy, may provide a more efficient gene therapy, applicable for eventual human implantation of gene transferred cells.

Original language	English (US)
Pages (from-to)	1259-1267
Number of pages	9
Journal	Peptides
Volume	25
Issue number	8
DOIs	https://doi.org/10.1016/j.peptides.2004.05.014
State	Published - Aug 2004

Bibliographical note

Funding Information:
National Nature Science Foundation of China (project 39970275, 30070278 for W.Z. and 30070198 for W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).

Keywords

ANP
CHF
CHO
CHO cells
Chinese hamster ovary
Chronobiology
Circadian rhythm
Congestive heart failure
Encapsulation
PCL
RIA
atrial natriuretic peptide
congestive heart failure
hANP
human atrial natriuretic peptide
polycaprolactone
radioimmunoassay

Access

10.1016/j.peptides.2004.05.014

Cite this

@article{1238c294ab20465fb41de3ab1271b481,

title = "In vitro circadian ANP secretion by gene transferring cells encapsulated in polycaprolactone tubes: Gene chronotherapy",

abstract = "A new insofar as chronobiologic therapeutic approach by atrial natriuretic peptide (ANP) for hypertension and/or congestive heart failure (CHF) is based on the release of ANP from ANP cDNA transfected Chinese Hamster Ovary (CHO) cells encapsulated in polycaprolactone (PCL) tubes. ANP secretion was maintained for at least 6 months. The encapsulated cells remained viable during culturing. Control cells without transferred ANP cDNA were negative. ANP secretion is circadian periodic, peaking around 04:18, shifted to around 07:56 by melatonin treatment. The encapsulation technique, based on principles of chronotherapy, may provide a more efficient gene therapy, applicable for eventual human implantation of gene transferred cells.",

keywords = "ANP, CHF, CHO, CHO cells, Chinese hamster ovary, Chronobiology, Circadian rhythm, Congestive heart failure, Encapsulation, PCL, RIA, atrial natriuretic peptide, congestive heart failure, hANP, human atrial natriuretic peptide, polycaprolactone, radioimmunoassay",

author = "Zhengrong Wang and Liguo Chen and Chaomin Wan and Yi Qu and Cornelissen-Guillaume, {Germaine G} and F. Halberg",

note = "Funding Information: National Nature Science Foundation of China (project 39970275, 30070278 for W.Z. and 30070198 for W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).",

year = "2004",

month = aug,

doi = "10.1016/j.peptides.2004.05.014",

language = "English (US)",

volume = "25",

pages = "1259--1267",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "8",

}

TY - JOUR

T1 - In vitro circadian ANP secretion by gene transferring cells encapsulated in polycaprolactone tubes

T2 - Gene chronotherapy

AU - Wang, Zhengrong

AU - Chen, Liguo

AU - Wan, Chaomin

AU - Qu, Yi

AU - Cornelissen-Guillaume, Germaine G

AU - Halberg, F.

N1 - Funding Information: National Nature Science Foundation of China (project 39970275, 30070278 for W.Z. and 30070198 for W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).

PY - 2004/8

Y1 - 2004/8

N2 - A new insofar as chronobiologic therapeutic approach by atrial natriuretic peptide (ANP) for hypertension and/or congestive heart failure (CHF) is based on the release of ANP from ANP cDNA transfected Chinese Hamster Ovary (CHO) cells encapsulated in polycaprolactone (PCL) tubes. ANP secretion was maintained for at least 6 months. The encapsulated cells remained viable during culturing. Control cells without transferred ANP cDNA were negative. ANP secretion is circadian periodic, peaking around 04:18, shifted to around 07:56 by melatonin treatment. The encapsulation technique, based on principles of chronotherapy, may provide a more efficient gene therapy, applicable for eventual human implantation of gene transferred cells.

AB - A new insofar as chronobiologic therapeutic approach by atrial natriuretic peptide (ANP) for hypertension and/or congestive heart failure (CHF) is based on the release of ANP from ANP cDNA transfected Chinese Hamster Ovary (CHO) cells encapsulated in polycaprolactone (PCL) tubes. ANP secretion was maintained for at least 6 months. The encapsulated cells remained viable during culturing. Control cells without transferred ANP cDNA were negative. ANP secretion is circadian periodic, peaking around 04:18, shifted to around 07:56 by melatonin treatment. The encapsulation technique, based on principles of chronotherapy, may provide a more efficient gene therapy, applicable for eventual human implantation of gene transferred cells.

KW - ANP

KW - CHF

KW - CHO

KW - CHO cells

KW - Chinese hamster ovary

KW - Chronobiology

KW - Circadian rhythm

KW - Congestive heart failure

KW - Encapsulation

KW - PCL

KW - RIA

KW - atrial natriuretic peptide

KW - congestive heart failure

KW - hANP

KW - human atrial natriuretic peptide

KW - polycaprolactone

KW - radioimmunoassay

UR - http://www.scopus.com/inward/record.url?scp=4444329333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444329333&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2004.05.014

DO - 10.1016/j.peptides.2004.05.014

M3 - Article

C2 - 15350693

AN - SCOPUS:4444329333

VL - 25

SP - 1259

EP - 1267

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 8

ER -

In vitro circadian ANP secretion by gene transferring cells encapsulated in polycaprolactone tubes: Gene chronotherapy

Abstract

Bibliographical note

Keywords

Access

Other files and links

Fingerprint

Cite this