In vitro cell response on CP-Ti surfaces functionalized with TGF-β1 inhibitory peptides

Pablo Sevilla, Andrea Cirera, Javier Dotor, Francisco Javier Gil, Pablo Galindo-Moreno, Conrado Aparicio

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13 Scopus citations


Osseointegration of implants is conversely related to the generation of a fibrous tissue capsule around the implant by the host environment. Although TGF-β1 plays many roles in regeneration processes, it is the cytokine to be mostly associated to the production of fibrotic tissue and thus, its inhibition has demonstrated to be beneficial to prevent several fibrotic reactions. Surface biofunctionalization enables the immobilization of biologically active molecules on an implant surface to tailor the biological response of the host. Here, we studied in vitro biological effects of biofunctionalized CP-Ti surfaces with a TGF-β1 inhibitor peptide, P144. A reliable biofunctionalization process that tethers P144 peptides to commercially pure titanium was developed. Differentiation of human mesenchymal stem cells, osteoblasts and fibroblasts on P144-functionalized and control surfaces was assessed at the gene expression and protein production levels. Results showed that P144-functionalized surfaces reduced expression and production of fibrotic differentiation markers and increased osteoblastic differentiation markers. Therefore, biofunctionalization of surfaces with TGF-β1 inhibitor peptides are an alternative promising strategy for inducing osseointegration around medical devices and implants.

Original languageEnglish (US)
Article number73
JournalJournal of Materials Science: Materials in Medicine
Issue number6
StatePublished - Jun 1 2018

Bibliographical note

Funding Information:
Acknowledgements This work was partially supported by the University of Minnesota through a Grant-in-Aid of Research, Artistry, and Scholarly (CA), and the Spanish Ministry of Economy and Competitiveness (project MAT 2012-30706) (FJG). PS acknowledges financial support from the Spanish Ministry of Economy and Competitiveness through a fellowship for research stays at international institutions (FPU-MEC). Parts of this work were carried out in the University of Minnesota I.T. Characterization Facility, which receives partial support from NSF through the MRSEC program. Parts of this work were carried out in the Institute for Bioengineering of Catalonia-IBEC.

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.


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