In vitro antifungal efficacy of four irrigants as a final rinse

Melissa L. Ruff, Scott B. McClanahan, Britta S. Babel

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


The purpose of this study was to compare the antifungal efficacy of 6% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX), 17% ethylene-diaminetetraacetic acid (EDTA), and BioPure MTAD as a final rinse on Candida albicans in vitro. Forty-eight, single rooted teeth were randomly divided into four groups each with a positive and a negative control. After root canal preparation, experimental teeth were inoculated with Candida albicans (ATCC 60193) and incubated for 72 hours. The groups were rinsed as follows: 1 ml of 6% NaOCl, 0.2 ml of 2% CHX, 1 ml of 17% EDTA, and 5 ml of BioPure MTAD. Aliquots from the experimental teeth were plated on Sabouraud 4% dextrose agar plates and colony-forming units were counted as a measure of antifungal activity. The results showed that 6% NaOCl and 2% CHX were equally effective and statistically significantly superior to BioPure MTAD and 17% EDTA (p < 0.05) in antifungal activity.

Original languageEnglish (US)
Pages (from-to)331-333
Number of pages3
JournalJournal of Endodontics
Issue number4
StatePublished - Apr 1 2006


  • Antifungal
  • Antimicrobial
  • BioPure MTAD
  • Candida albicans
  • Chlorhexidine gluconate
  • ethylenediaminetetraacetic acid (EDTA)
  • irrigants
  • smear layer
  • sodium hypochlorite


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