In vitro and in vivo suppression of cellular activity by guanidinoethyl disulfide released from hydrogel microspheres composed of partially oxidized hyaluronan and gelatin

Lihui Weng, Natalia D. Ivanova, Julia Zakhaleva, Weiliam Chen

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12 Scopus citations

Abstract

This paper describes the preparation of oxidized hyaluronan crosslinked gelatin microspheres for drug delivery. Microspheres were prepared by a modified water-in-oil-emulsion crosslinking method, where three-dimensional crosslinked hydrogel microspheres formed in the absence of any extraneous crosslinker. SEM analyses of the microspheres showed rough surfaces in their dried state with an average diameter of 90 μm. Lyophilization of fully swollen microspheres revealed a highly porous structure. Guanidinoethyl disulfide (GED) was used as a model drug for incorporation into the microspheres; encapsulation of GED was confirmed by HPLC. There was an inverse correlation between the diameters of the microspheres with their GED loading. Macrophage was used as a model cell to evaluate the in vitro efficacy of GED release from the microspheres. The in vivo efficacy of the microspheres was further validated in a mouse full-thickness transcutaneous dermal wound model through suppression of cell infiltration.

Original languageEnglish (US)
Pages (from-to)4149-4156
Number of pages8
JournalBiomaterials
Volume29
Issue number31
DOIs
StatePublished - Nov 2008

Bibliographical note

Funding Information:
Funding of this study by the National Institutes of Health (DK068401, WC) is gratefully acknowledged. We would also like to thank Jim Quinn in the Department of Material Science and Engineering (SUNY-SB) for the SEM analysis.

Keywords

  • Cell infiltration
  • Gelatin
  • Guanidinoethyl disulfide
  • Hyaluronan
  • Microspheres

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