In vitro and in vivo evidence for oxalate oxidase activity of a germin-like protein from azalea

Atsushi Sakamoto; Takashi Nishimura; Yoh Ichi Miyaki; Shunsuke Watanabe; Hiroshi Takagi; Shunsuke Izumi; Hiroshi Shimada

doi:10.1016/j.bbrc.2015.02.002

In vitro and in vivo evidence for oxalate oxidase activity of a germin-like protein from azalea

Atsushi Sakamoto, Takashi Nishimura, Yoh Ichi Miyaki, Shunsuke Watanabe, Hiroshi Takagi, Shunsuke Izumi, Hiroshi Shimada

Plant and Microbial Biology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Germins and germin-like proteins (GLPs) comprise large families of extracellular plant glycoproteins that are structurally similar, yet they have been reported to have distinct biochemical activities: oxalate oxidase and superoxide dismutase activities, respectively. We expressed an azalea GLP (RmGLP2) in cultured cells of tobacco, and determined that the extracellular protein fraction and the recombinant RmGLP2 protein purified from these cells catalyzed the oxidation of oxalate. Notably, this activity is purportedly restricted to germin and has not been demonstrated for a GLP. Although the specific activity of the purified RmGLP2 protein was low compared with that of a previously characterized barley germin/oxalate oxidase, tobacco cells expressing RmGLP2 exhibited significantly reduced oxalate levels. Thus, RmGLP2 represents the first reported GLP with oxalate oxidase activity.

Original language	English (US)
Pages (from-to)	536-542
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	458
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2015.02.002
State	Published - Mar 13 2015

Bibliographical note

Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research on Innovative Areas (grant nos. 23119514 and 25119717 to A.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan .

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Keywords

Cupin
Extracellular matrix protein
Germin-like protein
Hydrogen peroxide
Oxalate oxidase
Superoxide dismutase

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title = "In vitro and in vivo evidence for oxalate oxidase activity of a germin-like protein from azalea",

abstract = "Germins and germin-like proteins (GLPs) comprise large families of extracellular plant glycoproteins that are structurally similar, yet they have been reported to have distinct biochemical activities: oxalate oxidase and superoxide dismutase activities, respectively. We expressed an azalea GLP (RmGLP2) in cultured cells of tobacco, and determined that the extracellular protein fraction and the recombinant RmGLP2 protein purified from these cells catalyzed the oxidation of oxalate. Notably, this activity is purportedly restricted to germin and has not been demonstrated for a GLP. Although the specific activity of the purified RmGLP2 protein was low compared with that of a previously characterized barley germin/oxalate oxidase, tobacco cells expressing RmGLP2 exhibited significantly reduced oxalate levels. Thus, RmGLP2 represents the first reported GLP with oxalate oxidase activity.",

keywords = "Cupin, Extracellular matrix protein, Germin-like protein, Hydrogen peroxide, Oxalate oxidase, Superoxide dismutase",

author = "Atsushi Sakamoto and Takashi Nishimura and Miyaki, {Yoh Ichi} and Shunsuke Watanabe and Hiroshi Takagi and Shunsuke Izumi and Hiroshi Shimada",

note = "Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Innovative Areas (grant nos. 23119514 and 25119717 to A.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan . Publisher Copyright: {\textcopyright} 2015 Elsevier Inc. All rights reserved.",

year = "2015",

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doi = "10.1016/j.bbrc.2015.02.002",

language = "English (US)",

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pages = "536--542",

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T1 - In vitro and in vivo evidence for oxalate oxidase activity of a germin-like protein from azalea

AU - Sakamoto, Atsushi

AU - Nishimura, Takashi

AU - Miyaki, Yoh Ichi

AU - Watanabe, Shunsuke

AU - Takagi, Hiroshi

AU - Izumi, Shunsuke

AU - Shimada, Hiroshi

N1 - Funding Information: This work was supported in part by Grants-in-Aid for Scientific Research on Innovative Areas (grant nos. 23119514 and 25119717 to A.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan . Publisher Copyright: © 2015 Elsevier Inc. All rights reserved.

PY - 2015/3/13

Y1 - 2015/3/13

N2 - Germins and germin-like proteins (GLPs) comprise large families of extracellular plant glycoproteins that are structurally similar, yet they have been reported to have distinct biochemical activities: oxalate oxidase and superoxide dismutase activities, respectively. We expressed an azalea GLP (RmGLP2) in cultured cells of tobacco, and determined that the extracellular protein fraction and the recombinant RmGLP2 protein purified from these cells catalyzed the oxidation of oxalate. Notably, this activity is purportedly restricted to germin and has not been demonstrated for a GLP. Although the specific activity of the purified RmGLP2 protein was low compared with that of a previously characterized barley germin/oxalate oxidase, tobacco cells expressing RmGLP2 exhibited significantly reduced oxalate levels. Thus, RmGLP2 represents the first reported GLP with oxalate oxidase activity.

AB - Germins and germin-like proteins (GLPs) comprise large families of extracellular plant glycoproteins that are structurally similar, yet they have been reported to have distinct biochemical activities: oxalate oxidase and superoxide dismutase activities, respectively. We expressed an azalea GLP (RmGLP2) in cultured cells of tobacco, and determined that the extracellular protein fraction and the recombinant RmGLP2 protein purified from these cells catalyzed the oxidation of oxalate. Notably, this activity is purportedly restricted to germin and has not been demonstrated for a GLP. Although the specific activity of the purified RmGLP2 protein was low compared with that of a previously characterized barley germin/oxalate oxidase, tobacco cells expressing RmGLP2 exhibited significantly reduced oxalate levels. Thus, RmGLP2 represents the first reported GLP with oxalate oxidase activity.

KW - Cupin

KW - Extracellular matrix protein

KW - Germin-like protein

KW - Hydrogen peroxide

KW - Oxalate oxidase

KW - Superoxide dismutase

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In vitro and in vivo evidence for oxalate oxidase activity of a germin-like protein from azalea

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