In vitro aggregation of the regulated secretory protein chromogranin A

Renu K. Jain, Wen Tzu Chang, Chitta Geetha, Paul B.M. Joyce, Sven Ulrik Gorr

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Aggregation chaperones, consisting of secretory proteins that contain a hexa-histidine epitope tag, enhance the calcium-induced aggregation of regulated secretory proteins and their sorting to secretory granules. The goal of this study was to gain a better understanding of this unusual aggregation mechanism. Hexahistidine-epitope-tagged secreted alkaline phosphatase, an aggregation chaperone, enhanced the in vitro aggregation of chromogranin A in the presence of calcium, but not in the presence of magnesium or other divalent cations. As an exception, chromogranin was completely aggregated by zinc, even in the absence of the aggregation chaperone. In addition, fluorescence spectroscopy of the aggregation reaction mixture showed an increase in fluorescence intensity consistent with the formation of protein aggregates. The calcium-induced aggregation of chromogranin A was completely inhibited by 0.2% Triton X-100, suggesting that it involves hydrophobic interactions. In contrast, the detergent did not affect chaperone-enhanced aggregation, suggesting that this aggregation does not depend on hydrophobic interactions. EDTA-treated chaperone did not enhance chromogranin A aggregation, indicating that divalent cations are necessary for chaperone action. Although the structure of the aggregation chaperone was not important, the size of the chaperone was. Thus the free His-hexapeptide could not substitute for the aggregation chaperone. Based on these results, we propose that the hexa-histidine tag, in the context of a polypeptide, acts as a divalent cation-dependent nucleation site for chromogranin A aggregation.

Original languageEnglish (US)
Pages (from-to)605-610
Number of pages6
JournalBiochemical Journal
Volume368
Issue number2
DOIs
StatePublished - Dec 1 2002

Keywords

  • Aggregation chaperone
  • Calcium
  • Hexa-histidine
  • His epitope tag
  • Secreted alkaline phosphatase

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