In vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci.

M. Laverdiere, P. K. Peterson, J. Verhoef, D. N. Williams, L. D. Sabath

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The in vitro activity of five cephalosporins, gentamicin, and vancomycin was determined against 41 clinical isolates of methicillin-resistant, coagulase-negative staphylococci. Results obtained with disk diffusion and agar dilution methods failed to show complete cross-resistance between methicillin and four of the five cephalosporins despite the use of a high-salt medium and a large inoculum. Thirty-six (88%) of the 41 isolates were sensitive to cephalothin by a standardized disk diffusion method, whereas 23 isolates (56%) were sensitive to cephalothin with use of an agar dilution method and a large inoculum. Of these 23 isolates, only 11 (47%) were both inhibited and killed by less than or equal to 6.25 microgram of cephalothin/ml. The inhibitory and bactericidal activity of gentamicin was greater than that of vancomycin and cephalothin. These results suggest that the disk diffusion technique has limitations in determining the in vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci and that cephalothin exhibits poor bactericidal activity against these same isolates.
Original languageUndefined/Unknown
Pages (from-to)245-250
Number of pages6
JournalJournal of Infectious Diseases
Volume137
Issue number3
StatePublished - 1978

Keywords

  • Cephalosporins/pd [Pharmacology]
  • Coagulase/me [Metabolism]
  • Gentamicins/pd [Pharmacology]
  • Methicillin/pd [Pharmacology]
  • Vancomycin/pd [Pharmacology]

Cite this

In vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci. / Laverdiere, M.; Peterson, P. K.; Verhoef, J.; Williams, D. N.; Sabath, L. D.

In: Journal of Infectious Diseases, Vol. 137, No. 3, 1978, p. 245-250.

Research output: Contribution to journalArticle

Laverdiere, M. ; Peterson, P. K. ; Verhoef, J. ; Williams, D. N. ; Sabath, L. D. / In vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci. In: Journal of Infectious Diseases. 1978 ; Vol. 137, No. 3. pp. 245-250.
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abstract = "The in vitro activity of five cephalosporins, gentamicin, and vancomycin was determined against 41 clinical isolates of methicillin-resistant, coagulase-negative staphylococci. Results obtained with disk diffusion and agar dilution methods failed to show complete cross-resistance between methicillin and four of the five cephalosporins despite the use of a high-salt medium and a large inoculum. Thirty-six (88{\%}) of the 41 isolates were sensitive to cephalothin by a standardized disk diffusion method, whereas 23 isolates (56{\%}) were sensitive to cephalothin with use of an agar dilution method and a large inoculum. Of these 23 isolates, only 11 (47{\%}) were both inhibited and killed by less than or equal to 6.25 microgram of cephalothin/ml. The inhibitory and bactericidal activity of gentamicin was greater than that of vancomycin and cephalothin. These results suggest that the disk diffusion technique has limitations in determining the in vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci and that cephalothin exhibits poor bactericidal activity against these same isolates.",
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T1 - In vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci.

AU - Laverdiere, M.

AU - Peterson, P. K.

AU - Verhoef, J.

AU - Williams, D. N.

AU - Sabath, L. D.

PY - 1978

Y1 - 1978

N2 - The in vitro activity of five cephalosporins, gentamicin, and vancomycin was determined against 41 clinical isolates of methicillin-resistant, coagulase-negative staphylococci. Results obtained with disk diffusion and agar dilution methods failed to show complete cross-resistance between methicillin and four of the five cephalosporins despite the use of a high-salt medium and a large inoculum. Thirty-six (88%) of the 41 isolates were sensitive to cephalothin by a standardized disk diffusion method, whereas 23 isolates (56%) were sensitive to cephalothin with use of an agar dilution method and a large inoculum. Of these 23 isolates, only 11 (47%) were both inhibited and killed by less than or equal to 6.25 microgram of cephalothin/ml. The inhibitory and bactericidal activity of gentamicin was greater than that of vancomycin and cephalothin. These results suggest that the disk diffusion technique has limitations in determining the in vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci and that cephalothin exhibits poor bactericidal activity against these same isolates.

AB - The in vitro activity of five cephalosporins, gentamicin, and vancomycin was determined against 41 clinical isolates of methicillin-resistant, coagulase-negative staphylococci. Results obtained with disk diffusion and agar dilution methods failed to show complete cross-resistance between methicillin and four of the five cephalosporins despite the use of a high-salt medium and a large inoculum. Thirty-six (88%) of the 41 isolates were sensitive to cephalothin by a standardized disk diffusion method, whereas 23 isolates (56%) were sensitive to cephalothin with use of an agar dilution method and a large inoculum. Of these 23 isolates, only 11 (47%) were both inhibited and killed by less than or equal to 6.25 microgram of cephalothin/ml. The inhibitory and bactericidal activity of gentamicin was greater than that of vancomycin and cephalothin. These results suggest that the disk diffusion technique has limitations in determining the in vitro activity of cephalosporins against methicillin-resistant, coagulase-negative staphylococci and that cephalothin exhibits poor bactericidal activity against these same isolates.

KW - Cephalosporins/pd [Pharmacology]

KW - Coagulase/me [Metabolism]

KW - Gentamicins/pd [Pharmacology]

KW - Methicillin/pd [Pharmacology]

KW - Vancomycin/pd [Pharmacology]

M3 - Article

VL - 137

SP - 245

EP - 250

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 3

ER -