In utero alcohol exposure increases mammary tumorigenesis in rats

L. Hilakivi-Clarke, A. Cabanes, S. De Assis, M. Wang, G. Khan, W. J. Shoemaker, R. G. Stevens

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Findings in humans and animal models suggest that in utero hormonal and dietary exposures increase later breast cancer risk. Since alcohol intake by adult women consistently increases their breast cancer risk, we wondered whether maternal alcohol consumption during pregnancy increases female offspring's mammary tumorigenesis. In our study, pregnant female rats were pair-fed isocaloric diets containing either 0 (control), 16 or 25 g alcohol kg -1 feed between days 7 and 19 of gestation. These alcohol exposures generate blood alcohol levels that correspond to low and moderate alcohol consumption and are lower than those that induce foetal alcohol syndrome. Serum oestradiol levels were elevated in pregnant rats exposed to alcohol (P < 0.003). When adult, female offspring of alcohol-exposed dams developed significantly more 7,12-dimethylbenz[a]anthracene -induced mammary tumours, compared to the controls (tumour multiplicity; mean ± s.e.m., controls: 2.0 ± 0.3, 16 g alcohol: 2.7 ± 0.4 and 25 g alcohol: 3.7 ± 0.4; P < 0.006). In addition, the mammary epithelial tree of the alcohol-exposed offspring was denser (P < 0.004) and contained more structures that are susceptible for the initiation of breast cancer (P < 0.001). Immunohistochemical assessment indicated that the mammary glands of 22-week-old in utero alcohol-exposed rats contained elevated levels of oestrogen receptor-α (P < 0.04) that is consistent with the changes in mammary gland morphology. In summary, maternal alcohol intake during pregnancy increases female offspring's mammary tumorigenesis, perhaps by programming the foetal mammary gland to exhibit persistent alterations in morphology and gene expression. It remains to be determined whether an increase in pregnancy oestradiol levels mediated alcohol's effects on offspring's mammary tumorigenesis.

Original languageEnglish (US)
Pages (from-to)2225-2231
Number of pages7
JournalBritish Journal of Cancer
Volume90
Issue number11
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

Bibliographical note

Funding Information:
We thank Aaron Foxhall for performing the animal studies, and Johan Clarke and Antonia Chezek for technical assistance. The study was supported by grants received from the Susan G Komen Breast Cancer Foundation and Breast Cancer Research Foundation (to L H-C).

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

  • Alcohol
  • Breast cancer
  • Mammary gland
  • Oestrogen receptor
  • in utero exposures

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