In situ thermal denaturation of proteins in dunning AT-1 prostate cancer cells: Implication for hyperthermic cell injury

Xiaoming He, Willem F. Wolkers, John H. Crowe, David J. Swanlund, John C. Bischof

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

The in-situ thermal protein denaturation and its correlation with direct hyperthermic cell injury in Dunning AT-1 prostate tumor cells were investigated. The in situ thermal protein denaturation was studied using both Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). The FTIR spectra at different temperatures show changes in protein secondary structure during in situ thermal protein denaturation within AT-1 cells. Calorimetric studies using DSC show that endothermic heat release is associated with the in situ thermal protein denaturation.

Original languageEnglish (US)
Pages (from-to)1384-1398
Number of pages15
JournalAnnals of Biomedical Engineering
Volume32
Issue number10
DOIs
StatePublished - Oct 2004

Bibliographical note

Funding Information:
Thanks to Urologix, Inc. (Minneapolis, MN), for their support of this study. This work was also partially supported by grants HL57810 and HL61204 from the NIH, and 981711 from DARPA to Dr Crowe at the University of California, Davis.

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

  • AT-1
  • Cell injury
  • DSC
  • Denaturation
  • FTIR
  • Kinetics
  • Mechanism
  • Modeling
  • Protein
  • Thermal injury

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