Rotavirus groups A, B, and C (RVA, RVB, and RVC, respectively) have been the most prevalent and pathogenic in pigs. To date, immunohistochemistry is only available for RVA because of the lack of commercial antibodies for RVB and RVC. We developed a novel in situ hybridization RNA-based chromogenic technique (ISH-RNA) to detect and subtype RVA, RVB, and RVC. We evaluated 33 samples that were reverse-transcription PCR positive for RVA, RVB, and/or RVC. ISH-RNA was able to detect as few as 10 3 RV RNA copies/mL. The new ISH-RNA test can be useful for routine investigation of rotavirus enteritis in order to guide strategies for control of the infection in pigs, but a full validation study needs to be completed. Pathogenesis studies may be conducted using ISH-RNA based on the identification of replicating virus.
Bibliographical noteFunding Information:
We thank all of the employees in the histology and immunohistochemistry laboratories of the University of Minnesota Veterinary Diagnostic Laboratory for their help during the ISH testing, and Lacey Marshal Lund for language assistance. TP Resende was supported by CAPES?Brazil. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
TP Resende was supported by CAPES–Brazil. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
© 2018 The Author(s).
- in situ hybridization
- viral enteritis