In situ analysis reveals physical interactions between CD11b+ dendritic cells and antigen-specific CD4 T cells after subcutaneous injection of antigen

Elizabeth Ingulli, Deborah R. Ulman, Michelle M. Lucido, Marc K. Jenkins

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

In situ staining techniques were used to visualize physical interactions between dendritic cell subsets and naive Ag-specific CD4 T cells in the lymph node. Before injection of Ag, CD8+ dendritic cells and naive OVA-specific CD4 T cells were uniformly distributed throughout the T cell-rich paracortex, whereas CD11b+ dendritic cells were located mainly in the outer edges of the paracortex near the B cell-rich follicles. Many OVA-specific CD4 T cells were in contact with CD8+ dendritic cells in the absence of OVA. Within 24 h after s.c. injection of soluble OVA, the OVA-specific CD4 T cells redistributed to the outer paracortex and interacted with CD11b+, but not CD8+ dendritic cells. This behavior correlated with the uptake of OVA and the presence of peptide-MHC complexes on the surface of CD11b+ dendritic cells, and subsequent IL-2 production by the Ag-specific CD4 T cells. These results are consistent with the possibility that CD11b+ dendritic cells play a central role in the activation of CD4 T cells in response to s.c. Ag.

Original languageEnglish (US)
Pages (from-to)2247-2252
Number of pages6
JournalJournal of Immunology
Volume169
Issue number5
DOIs
StatePublished - Sep 1 2002

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