In Silico Prediction of MYO1C-Rhodopsin Interactions and Its Significance in Protein Localization and Visual Function

Glenn P. Lobo, Rakesh Radhakrishnan, Matthias M Leung, Andrew Gruesen, Hans Joachim Knölker, Frederik J. van Kuijk, Sandra R. Montezuma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Rods and cones are photoreceptor neurons in the retina that are required for visual sensation in vertebrates, where proper protein localization and compartmentalization are critical for phototransduction and visual function. In human retinal diseases, improper protein transport to the outer segment (OS) or mislocalization of proteins to the inner segment (IS) could lead to impaired visual responses and photoreceptor cell degeneration, causing a loss of visual function. We showed involvement of an unconventional motor protein, MYO1C, in the proper localization of rhodopsin to the OS, where loss of MYO1C in a mammalian model caused mislocalization of rhodopsin to IS and cell bodies, leading to progressively severe retinal phenotypes. In this study, using modeling and docking analysis, we aimed to identify the protein–protein interaction sites between MYO1C and Rhodopsin to establish a hypothesis that a physical interaction between these proteins is necessary for the proper trafficking of rhodopsin and visual function.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer
Pages499-505
Number of pages7
ISBN (Print)9783031276804, 9783031276811
DOIs
StatePublished - 2023

Publication series

NameAdvances in experimental medicine and biology
PublisherSpringer New York
ISSN (Print)0065-2598

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the National Institute of Health—National Eye Institute (NIH-NEI) grants R21EY025034 and R01EY030889 to G.P.L.T his project was also supported in part by a SCTR-NIH/NCATS grant (5UL1TR001450) and University of Minnesota Start-up funds to G.P.L.

Funding Information:
This work was supported by the National Institute of Health—National Eye Institute (NIH-NEI) grants R21EY025034 and R01EY030889 to G.P.L. This project was also supported in part by a SCTRNIH/NCATS grant (5UL1TR001450) and University of Minnesota Start-up funds to G.P.L.

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

Keywords

  • Motor protein
  • Myosin 1C
  • Outer segments
  • Photoreceptor
  • Protein localization
  • Retina
  • Rhodopsin
  • Visual function
  • Humans
  • Protein Transport/physiology
  • Rhodopsin/genetics
  • Retinal Rod Photoreceptor Cells/metabolism
  • Animals
  • Mammals/metabolism
  • Retina/metabolism
  • Retinal Cone Photoreceptor Cells/metabolism
  • Myosin Type I/metabolism

PubMed: MeSH publication types

  • Journal Article

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