Improving sensitivity in micro-free flow electrophoresis using signal averaging

Ryan T. Turgeon, Michael T. Bowser

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Microfluidic free-flow electrophoresis (μFFE) is a separation technique that separates continuous streams of analytes as they travel through an electric field in a planar flow channel. The continuous nature of the μFFE separation suggests that approaches more commonly applied in spectroscopy and imaging may be effective in improving sensitivity. The current paper describes the S/N improvements that can be achieved by simply averaging multiple images of a μFFE separation; 20-24-fold improvements in S/N were observed by averaging the signal from 500 images recorded for over 2 min. Up to an 80-fold improvement in S/N was observed by averaging 6500 images. Detection limits as low as 14 pM were achieved for fluorescein, which is impressive considering the nonideal optical set-up used in these experiments. The limitation to this signal averaging approach was the stability of the μFFE separation. At separation times longer than 20 min bubbles began to form at the electrodes, which disrupted the flow profile through the device, giving rise to erratic peak positions.

Original languageEnglish (US)
Pages (from-to)1342-1348
Number of pages7
JournalELECTROPHORESIS
Volume30
Issue number8
DOIs
StatePublished - 2009

Keywords

  • Free-flow electrophoresis
  • LIF
  • Signal averaging

Fingerprint Dive into the research topics of 'Improving sensitivity in micro-free flow electrophoresis using signal averaging'. Together they form a unique fingerprint.

Cite this