Improving hydrogen and methane co-generation in cascading dark fermentation and anaerobic digestion: The effect of magnetite nanoparticles on microbial electron transfer and syntrophism

Jun Cheng, Hui Li, Lingkan Ding, Junhu Zhou, Wenlu Song, Yu You Li, Richen Lin

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146 Scopus citations

Abstract

The efficiency of microbial electron transfer is fundamental for determining the performance of fermentative hydrogen/methane production. To facilitate microbial electron transfer, conductive magnetite nanoparticles (MNPs) were added into a cascading dark fermentation and anaerobic digestion system that was inoculated with Enterobacter aerogenes ZJU1 and methanogenic activated sludge (MAS), respectively. During the hydrogen-producing stage, the ratio of NADH/NAD+ and the activities of hydrogenase and electron transport system (ETS) of E. aerogenes ZJU1 were all increased by dosing 200 mg/L MNPs, which was conducive to hydrogen production through the NADH-dependent pathway. In the presence of 200 mg/L MNPs, hydrogen production increased by 21.1%, while subsequent methane production improved by 22.9%. Electrochemical analysis demonstrated the improvement in extracellular electron transfer capacity of MAS after adding MNPs, which can be ascribed to the contribution of MNPs and electrochemically active extracellular polymeric substances (EPS) induced by MNPs, such as humic acid-like and fulvic acid-like substances. Bacteria Syntrophomonas and Archaea Methanosarcina were the dominating enriched syntrophic partners, and the expression of functional genes involved in CO2 reduction to methane pathway was found to increase. Therefore, a more efficient fermentative hydrogen and methane co-production system was established by improving microbial electron transfer with the addition of MNPs.

Original languageEnglish (US)
Article number125394
JournalChemical Engineering Journal
Volume397
DOIs
StatePublished - Oct 1 2020

Bibliographical note

Funding Information:
This study was supported by the National Key Research and Development Program-China ( 2016YFE0117900 ), and Zhejiang Provincial Key Research and Development Program-China ( 2017C04001 ). Dr. Wenlu Song acknowledges supports from Shandong Province Higher Educational Science and Technology Program ( J17KA095 ), Shandong Provincial Natural Science Foundation ( ZR2019MC060 ) and Key Research and Development Program of Jining City ( 2018ZDGH024 ). In addition, Dr. Richen Lin acknowledges support from the European Union’s Marie Skłodowska-Curie grant (No. 797259 ).

Funding Information:
This study was supported by the National Key Research and Development Program-China (2016YFE0117900), and Zhejiang Provincial Key Research and Development Program-China (2017C04001). Dr. Wenlu Song acknowledges supports from Shandong Province Higher Educational Science and Technology Program (J17KA095), Shandong Provincial Natural Science Foundation (ZR2019MC060) and Key Research and Development Program of Jining City (2018ZDGH024). In addition, Dr. Richen Lin acknowledges support from the European Union's Marie Sk?odowska-Curie grant (No. 797259).

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

  • Anaerobic digestion (AD)
  • Dark fermentation
  • Direct interspecies electron transfer (DIET)
  • Electron transport system (ETS)
  • Hydrogenase
  • Magnetite nanoparticles

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