Improvements for the Micropropagation of Hybrid Hazelnut (C. americana × C. avellana)

Renata P. Pincelli-Souza, Laise Sousa Moreira, Jerry D. Cohen

Research output: Contribution to journalArticlepeer-review


The micropropagation of hybrid hazelnut (Corylus americana × Corylus avellana) has been limited in its agricultural application due to the lack of efficient procedures for tissue culture and root organogenesis. We established an efficient sterilization protocol for hazelnut micropropagation suitable for in vitro root induction that permitted us to approach the poor root organogenesis experienced with the micropropagation of hazelnut hybrids in vitro. Typically, with standard protocols, root organogenesis had yields of well under 20%. We found that cuttings grown in vitro to the four-leaf stage can be successfully rooted using a combination of indole-3-butyric acid (IBA) pretreatment, followed by a one-week growth period in absolute darkness. The dark period was then followed by a transition to continuous light. In many cases, prolific visible roots formed between 12 and 14 days.

Original languageEnglish (US)
Article number849
Issue number9
StatePublished - Sep 2022

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation (NSF) Plant Genome Research Program [grant IOS-1238812], the US Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) [No. 2018-67013-27503] and USDA [SCRI 2019-51181-30025], Minnesota Department of Agriculture [grant 92336_2021 SCBG] and by funds from the Minnesota Agricultural Experiment Station and the Gordon and Margaret Bailey Endowment for Environmental Horticulture.

Publisher Copyright:
© 2022 by the authors.


  • dark/light treatments
  • filbert nut
  • organogenesis
  • photomorphogenesis
  • root initiation


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