TY - JOUR
T1 - Improved vectors for nisin-controlled expression in gram-positive bacteria
AU - Bryan, Edward M.
AU - Bae, Taeok
AU - Kleerebezem, Michiel
AU - Dunny, Gary M.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - A set of shuttle vectors, able to replicate in Escherichia coli and in gram-positive bacteria, containing a nisin-inducible promoter (PnisA) and genes encoding NisR and NisK, the two-component signaling mechanism for activating transcription from PnisA in the presence of nisin, was constructed. To test these vectors, Enterococcus faecalis pCF10 plasmid genes prgX, prgY, and prgZ, which respectively encode cytosolic, integral membrane, and cell surface proteins, were cloned downstream of PnisA. Increased protein expression, in the presence of nisin, was demonstrated by Western blot analysis. (C) 2000 Academic Press.
AB - A set of shuttle vectors, able to replicate in Escherichia coli and in gram-positive bacteria, containing a nisin-inducible promoter (PnisA) and genes encoding NisR and NisK, the two-component signaling mechanism for activating transcription from PnisA in the presence of nisin, was constructed. To test these vectors, Enterococcus faecalis pCF10 plasmid genes prgX, prgY, and prgZ, which respectively encode cytosolic, integral membrane, and cell surface proteins, were cloned downstream of PnisA. Increased protein expression, in the presence of nisin, was demonstrated by Western blot analysis. (C) 2000 Academic Press.
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U2 - 10.1006/plas.2000.1484
DO - 10.1006/plas.2000.1484
M3 - Article
C2 - 10964628
AN - SCOPUS:0033812734
SN - 0147-619X
VL - 44
SP - 183
EP - 190
JO - Plasmid
JF - Plasmid
IS - 2
ER -