Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

Jenny N. Vo; Nur Nabihah Mahfuz; Benham E.L. Lockhart; Andrew D.W. Geering

doi:10.1007/s10658-015-0708-6

Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

Jenny N. Vo, Nur Nabihah Mahfuz, Benham E.L. Lockhart, Andrew D.W. Geering

Plant Pathology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The purification of viruses from bananas is a complex and laborious process due to the large quantities of polysaccharides and secondary metabolites in the plant tissue. The banana streak viruses (BSVs) are generally difficult to purify to a level that allows generation of high quality antisera. This study presents an improved method of purification of Banana streak MY virus (BSMYV), which allowed us to generate highly specific chicken and rabbit antisera and to detect the capsid proteins for the first time. Finally, we demonstrate further enrichment of BSMYV by isoelectric focusing.

Original language	English (US)
Pages (from-to)	619-626
Number of pages	8
Journal	European Journal of Plant Pathology
Volume	143
Issue number	3
DOIs	https://doi.org/10.1007/s10658-015-0708-6
State	Published - Nov 1 2015

Bibliographical note

Publisher Copyright:
© 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.

Keywords

Badnavirus
Immunoassay
Liquid-phase-IEF
Musa
Polyclonal antibodies

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10.1007/s10658-015-0708-6

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abstract = "The purification of viruses from bananas is a complex and laborious process due to the large quantities of polysaccharides and secondary metabolites in the plant tissue. The banana streak viruses (BSVs) are generally difficult to purify to a level that allows generation of high quality antisera. This study presents an improved method of purification of Banana streak MY virus (BSMYV), which allowed us to generate highly specific chicken and rabbit antisera and to detect the capsid proteins for the first time. Finally, we demonstrate further enrichment of BSMYV by isoelectric focusing.",

keywords = "Badnavirus, Immunoassay, Liquid-phase-IEF, Musa, Polyclonal antibodies",

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T1 - Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

AU - Vo, Jenny N.

AU - Mahfuz, Nur Nabihah

AU - Lockhart, Benham E.L.

AU - Geering, Andrew D.W.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - The purification of viruses from bananas is a complex and laborious process due to the large quantities of polysaccharides and secondary metabolites in the plant tissue. The banana streak viruses (BSVs) are generally difficult to purify to a level that allows generation of high quality antisera. This study presents an improved method of purification of Banana streak MY virus (BSMYV), which allowed us to generate highly specific chicken and rabbit antisera and to detect the capsid proteins for the first time. Finally, we demonstrate further enrichment of BSMYV by isoelectric focusing.

AB - The purification of viruses from bananas is a complex and laborious process due to the large quantities of polysaccharides and secondary metabolites in the plant tissue. The banana streak viruses (BSVs) are generally difficult to purify to a level that allows generation of high quality antisera. This study presents an improved method of purification of Banana streak MY virus (BSMYV), which allowed us to generate highly specific chicken and rabbit antisera and to detect the capsid proteins for the first time. Finally, we demonstrate further enrichment of BSMYV by isoelectric focusing.

KW - Badnavirus

KW - Immunoassay

KW - Liquid-phase-IEF

KW - Musa

KW - Polyclonal antibodies

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M3 - Article

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JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

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Improved methods for the purification and enrichment of banana streak virus for antibody production and protein detection

Abstract

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