TY - JOUR
T1 - Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome
AU - Anazawa, T.
AU - Balamurugan, A. N.
AU - Papas, K. K.
AU - Avgoustiniatos, E. S.
AU - Ferrer, J.
AU - Matsumoto, S.
AU - Sutherland, D. E.R.
AU - Hering, B. J.
N1 - Funding Information:
Supported in part by grants from the Juvenile Diabetes Research Foundation (JDRF PPG #21-2006-881 ) and the National Institutes of Health ( U19 AI067151 ).
PY - 2010/7
Y1 - 2010/7
N2 - Background: Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of the pancreas by implementing a vascular flush and ductal preservation. Method: Pancreata procured from adult Landrace pigs were divided into 3 different surgical protocols: Pancreatectomy utilizing only surface cooling (group 1; n = 24); surface cooling and ductal injection with cold preservation solution before pancreatectomy (group 2; n = 12); or surface cooling, ductal injection, and an approach by selectively flushing through the celiac trunk and the superior mesenteric artery (group 3; n = 14). We assessed the islet isolation results and quality using in vitro and in vivo assays. Results: Significantly higher overall yield and islet yield per gram pancreas were obtained from group 3 pigs compared with the other groups. Measurements of islet viability after 7 days of culture, as assessed by oxygen consumption rate per DNA, showed that group 3 islets displayed the highest values. Sustained normoglycemia was observed in diabetic nude mice transplanted with 2000 islet equivalents from all 3 groups. Discussion: This study demonstrated that an advanced pancreas procurement technique including ductal preservation and selective arterial flush with cold preservation solution provided significant improvements in porcine islet isolation outcomes.
AB - Background: Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of the pancreas by implementing a vascular flush and ductal preservation. Method: Pancreata procured from adult Landrace pigs were divided into 3 different surgical protocols: Pancreatectomy utilizing only surface cooling (group 1; n = 24); surface cooling and ductal injection with cold preservation solution before pancreatectomy (group 2; n = 12); or surface cooling, ductal injection, and an approach by selectively flushing through the celiac trunk and the superior mesenteric artery (group 3; n = 14). We assessed the islet isolation results and quality using in vitro and in vivo assays. Results: Significantly higher overall yield and islet yield per gram pancreas were obtained from group 3 pigs compared with the other groups. Measurements of islet viability after 7 days of culture, as assessed by oxygen consumption rate per DNA, showed that group 3 islets displayed the highest values. Sustained normoglycemia was observed in diabetic nude mice transplanted with 2000 islet equivalents from all 3 groups. Discussion: This study demonstrated that an advanced pancreas procurement technique including ductal preservation and selective arterial flush with cold preservation solution provided significant improvements in porcine islet isolation outcomes.
UR - http://www.scopus.com/inward/record.url?scp=77955547789&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955547789&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2010.05.110
DO - 10.1016/j.transproceed.2010.05.110
M3 - Article
C2 - 20692400
AN - SCOPUS:77955547789
SN - 0041-1345
VL - 42
SP - 2032
EP - 2035
JO - Transplantation proceedings
JF - Transplantation proceedings
IS - 6
ER -