Improved measurement of 15 N-{ 1 H} NOEs in the presence of H(N)-water proton chemical exchange

Djaudat S Idiyatullin, Vladimir A. Daragan, Kevin H Mayo

Research output: Contribution to journalEditorialpeer-review

12 Scopus citations

Abstract

A simple method is presented to accurately determine 15 N-{ 1 H} NOEs in biomolecules in the presence of H(N)-water proton chemical exchange. Three measurements are required: one with nonselective proton saturation and two with different water saturation conditions to determine the equilibrium value of the 15 N signal. This approach is exemplified with data on two peptides, one helix-forming 17-mer and one compactly folded 56-mer. Results indicate that 15 N-{ 1 H} NOEs determined using the standard approach with short recycle times (3 to 4 s) can be significantly in error when H(N)-water proton chemical exchange is relatively rapid, water proton relaxation is relatively slow, and 15 N-{ 1 H} NOEs are away from the value of -1. This new method avoids such inaccuracies resulting from the use of short recycle times.

Original languageEnglish (US)
Pages (from-to)138-143
Number of pages6
JournalJournal of Magnetic Resonance
Volume153
Issue number1
DOIs
StatePublished - Jan 1 2001

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