Improved growth of bait microalgae Isochrysis and aquacultural wastewater treatment with mixotrophic culture

Ruirui Chu, Jingxue Ma, Chengxu Zhou, Defu Liu, Guangce Wang, Roger Ruan, Yandu Lu, Xiaojun Yan, Pengfei Cheng

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


This research of mixotrophic microalgae Isochrysis 3011 with glycerol was combined with the treatment of aqua-cultural wastewater, different initial concentrations, and optimized light intensities. The algae growth rate, removal efficiencies of total nitrogen (TN) and total phosphorus (TP) were determined. Results showed that the suitable initial concentration was 0.4 g L−1, and the optimum light intensity was 60 µmol m−2 s−1. The growth of the mixotrophic group was better than that of the autotrophic culture. The biomass yield of the mixotrophic group with glycerol was 0.17 g L−1 d−1, and the removal rates of TN and TP were 73.39% and 95.61%, respectively. The content of total lipid and total protein in mixotrophic group were higher than the values of the autotrophic group. This indicates that aquaculture wastewater treatment with mixotrophic bait microalgae can obtain superior micro-algal biomass, which is also a potential technology for wastewater utilization and ecological protection.

Original languageEnglish (US)
Pages (from-to)589-597
Number of pages9
JournalBioprocess and Biosystems Engineering
Issue number3
StatePublished - Mar 2022

Bibliographical note

Funding Information:
This research was supported in part by grants from the National Key Research and Development Program of China (2018YFD0901504), the National Natural Science Foundation of China (32170369), the State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University) (MRUKF2021003), the Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences (KF2019NO3), the Natural Science Foundation of Zhejiang Province (LY20D060003), the Fundamental Research Funds for the Provincial Universities (SJLY2020007), Ningbo Science and Technology Research Projects, China (2019C10023), the General Research Project of Zhejiang Provincial Department of Education (Y202045551), the Scientific Research Foundation of Graduate School of Ningbo University (IF2021081), and the Ningbo Municipal Science and Technology Project (2019C10071).

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.


  • Aquaculture wastewater
  • Bait microalgae
  • Mixotrophy
  • Phototrophy
  • Removal efficiency

PubMed: MeSH publication types

  • Journal Article


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