Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization

Sebastian Lücker, Doris Steger, Kasper Urup Kjeldsen, Barbara J. MacGregor, Michael Wagner, Alexander Loy

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes.

Original languageEnglish (US)
Pages (from-to)523-528
Number of pages6
JournalJournal of Microbiological Methods
Volume69
Issue number3
DOIs
StatePublished - Jun 1 2007

    Fingerprint

Keywords

  • 16S rRNA-targeted probes
  • Deltaproteobacteria
  • Fluorescence in situ hybridization
  • Sulfate-reducing bacteria

Cite this