Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization

Sebastian Lücker, Doris Steger, Kasper Urup Kjeldsen, Barbara J. MacGregor, Michael Wagner, Alexander Loy

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76 Scopus citations

Abstract

An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes.

Original languageEnglish (US)
Pages (from-to)523-528
Number of pages6
JournalJournal of Microbiological Methods
Volume69
Issue number3
DOIs
StatePublished - Jun 2007

Bibliographical note

Funding Information:
The authors thank Kjeld Ingvorsen for provision of the strains. Kilian Stoecker and Frank Maixner are acknowledged for excellent help with confocal laser scanning microscopy and for updating probeBase, respectively. This study was supported by grants from the European Community (Marie Curie Intra-European Fellowship within the 6th Framework Programme) and the Fonds zur Förderung der wissenschaftlichen Forschung (project P18836-B17) to AL, and the bmb+f (project 01 LC 0021A-TP2 in the framework of the BIOLOG II program) to MW.

Keywords

  • 16S rRNA-targeted probes
  • Deltaproteobacteria
  • Fluorescence in situ hybridization
  • Sulfate-reducing bacteria

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