Importance of conserved acidic residues in MntH, the Nramp homolog of Escherichia coli

H. A H Haemig, Robert J Brooker

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A bioinformatic approach was used for the identification of residues that are conserved within the Nramp family of metal transporters. Site-directed mutagenesis was then carried out to change six conserved acidic residues (i.e., Asp-34, Glu-102, Asp-109, Glu-112, Glu-154, and Asp-238) in the E. coli Nramp homolog mntH. Of these six, five of them, Asp-34, Glu-102, Asp-109, Glu-112, and Asp-238 appear to be important for function since conservative substitutions at these sites result in a substantial loss of transport function. In addition, all of the residues within the signature sequence of the Nramp family, DPGN, were also mutated in this study. Each residue was changed to several different side chains, and of ten site-directed mutations made in this motif, only P35G showed any measurable level of 54Mn2+ uptake with a V max value of approximately 10% of wild-type and a slightly elevated Km value. Overall, the data are consistent with a model where helix breakers in the conserved DPGN motif in TMS-1 provide a binding pocket in which Asp-34, Asn-37, Asp-109, Glu-112 (and possibly other residues) are involved in the coordination of Mn2+. Other residues such as Glu-102 and Asp238 may play a role in the release of Mn2+ to the cytoplasm or may be involved in maintaining secondary structure.

Original languageEnglish (US)
Pages (from-to)97-107
Number of pages11
JournalJournal of Membrane Biology
Volume201
Issue number2
DOIs
StatePublished - Nov 1 2004

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Site-Directed Mutagenesis
Computational Biology
Cytoplasm
Metals
Escherichia coli
Mutation
polyurethane-polypropylene composite

Keywords

  • Manganese transport
  • Nramp
  • Symporter

Cite this

Importance of conserved acidic residues in MntH, the Nramp homolog of Escherichia coli. / Haemig, H. A H; Brooker, Robert J.

In: Journal of Membrane Biology, Vol. 201, No. 2, 01.11.2004, p. 97-107.

Research output: Contribution to journalArticle

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