Implementation of neutral loss experiments in the characterization of the Arabidopsis thaliana phosphorylome by one and two dimensional LC ESI Q-TOF MS/MS

Clark J. Nelson, Adrian D. Hegeman, Amy C. Harms, Michael R. Sussman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The Arabidopsis thaliana phosphorylome was characterized by the implementation of neutral loss experiments and one and two dimensional LC ESI Q-TOF MS/MS. Lyophilized peptides were resuspended in 2M methanolic HCl and allowed to react for two hours at room temperature. One-dimensional analysis of peptides was conducted on pulled-tip fused silica capillary columns packed with 10cm of RP resin. The results show that the identification of phosphorylation sites can be used in the investigation of signal transduction and cellular regulation.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages235-236
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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