Implementation of neutral loss experiments in the characterization of the Arabidopsis thaliana phosphorylome by one and two dimensional LC ESI Q-TOF MS/MS

Clark J. Nelson; Adrian D. Hegeman; Amy C. Harms; Michael R. Sussman

Implementation of neutral loss experiments in the characterization of the Arabidopsis thaliana phosphorylome by one and two dimensional LC ESI Q-TOF MS/MS

Clark J. Nelson, Adrian D. Hegeman, Amy C. Harms, Michael R. Sussman

Horticultural Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The Arabidopsis thaliana phosphorylome was characterized by the implementation of neutral loss experiments and one and two dimensional LC ESI Q-TOF MS/MS. Lyophilized peptides were resuspended in 2M methanolic HCl and allowed to react for two hours at room temperature. One-dimensional analysis of peptides was conducted on pulled-tip fused silica capillary columns packed with 10cm of RP resin. The results show that the identification of phosphorylation sites can be used in the investigation of signal transduction and cellular regulation.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	235-236
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

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Implementation of neutral loss experiments in the characterization of the Arabidopsis thaliana phosphorylome by one and two dimensional LC ESI Q-TOF MS/MS. / Nelson, Clark J.; Hegeman, Adrian D.; Harms, Amy C. et al.
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 235-236.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nelson, CJ, Hegeman, AD, Harms, AC & Sussman, MR 2002, Implementation of neutral loss experiments in the characterization of the Arabidopsis thaliana phosphorylome by one and two dimensional LC ESI Q-TOF MS/MS. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 235-236, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

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abstract = "The Arabidopsis thaliana phosphorylome was characterized by the implementation of neutral loss experiments and one and two dimensional LC ESI Q-TOF MS/MS. Lyophilized peptides were resuspended in 2M methanolic HCl and allowed to react for two hours at room temperature. One-dimensional analysis of peptides was conducted on pulled-tip fused silica capillary columns packed with 10cm of RP resin. The results show that the identification of phosphorylation sites can be used in the investigation of signal transduction and cellular regulation.",

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year = "2002",

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AU - Sussman, Michael R.

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AB - The Arabidopsis thaliana phosphorylome was characterized by the implementation of neutral loss experiments and one and two dimensional LC ESI Q-TOF MS/MS. Lyophilized peptides were resuspended in 2M methanolic HCl and allowed to react for two hours at room temperature. One-dimensional analysis of peptides was conducted on pulled-tip fused silica capillary columns packed with 10cm of RP resin. The results show that the identification of phosphorylation sites can be used in the investigation of signal transduction and cellular regulation.

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M3 - Conference contribution

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BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

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Y2 - 2 June 2002 through 6 June 2002

ER -

Implementation of neutral loss experiments in the characterization of the Arabidopsis thaliana phosphorylome by one and two dimensional LC ESI Q-TOF MS/MS

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