Implementation of a Diagnostic-Concentration Bioassay for Detection of Susceptibility to Pyrethroids in Soybean Aphid (Hemiptera: Aphididae)

James Menger, Patrick Beauzay, Anitha Chirumamilla, Cole Dierks, John Gavloski, Phillip A Glogoza, Krista Hamilton, Erin W. Hodgson, Janet J. Knodel, Ian V. MacRae, Daniela T. Pezzini, Bruce D Potter, Adam J. Varenhorst, Robert L. Koch, Troy Anderson

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Soybean aphid, Aphis glycines Matsumura, remains the most economically damaging arthropod pest of soybean in the midwestern United States and southern Canada. Foliar applications of a limited number of insecticide modes of action have been the primary management tactic, and pyrethroid resistance was documented recently with full concentration-response leaf-dip and glass-vial bioassays. Full concentration-response bioassays can be cumbersome, and a more efficient assessment tool was needed. In this study, we implemented a diagnostic-concentration glass-vial bioassay using bifenthrin and λ-cyhalothrin. Bioassays were conducted with field-collected soybean aphid populations to assess the geographic extent and severity of resistance to pyrethroids. In 2017, 10 of 18 and 11 of 21 field populations tested with bifenthrin and λ-cyhalothrin, respectively, had mean proportion mortalities less than the susceptible laboratory population. In 2018, 17 of 23 and 13 of 23 field populations tested with bifenthrin and λ-cyhalothrin, respectively, had mean proportion mortalities less than the susceptible laboratory population. Populations collected after reported field failures of a pyrethroid insecticide generally had mean proportion mortalities less than the susceptible laboratory population. In both years, there was a strong correlation between chemistries, which suggests cross-resistance between these insecticides. The diagnostic-concentration glass-vial bioassays reported here will provide the foundation for an insecticide resistance monitoring program with the ability to determine practical levels and geographic extent of insecticide resistance.

Original languageEnglish (US)
Pages (from-to)932-939
Number of pages8
JournalJournal of economic entomology
Volume113
Issue number2
DOIs
StatePublished - Apr 6 2020

Keywords

  • bifenthrin
  • bioassay
  • cross-resistance
  • glass-vial
  • λ-cyhalothrin

PubMed: MeSH publication types

  • Journal Article

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    Menger, J., Beauzay, P., Chirumamilla, A., Dierks, C., Gavloski, J., Glogoza, P. A., Hamilton, K., Hodgson, E. W., Knodel, J. J., MacRae, I. V., Pezzini, D. T., Potter, B. D., Varenhorst, A. J., Koch, R. L., & Anderson, T. (2020). Implementation of a Diagnostic-Concentration Bioassay for Detection of Susceptibility to Pyrethroids in Soybean Aphid (Hemiptera: Aphididae). Journal of economic entomology, 113(2), 932-939. https://doi.org/10.1093/jee/toz351